RHOT2

Protein identityUniProt · HPA

UniProt accession

Q8IXI1

Gene

RHOT2

Ensembl

ENSG00000140983

Chromosome

16

Canonical length

618 aa

Protein class

Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Mitochondria

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a member of the Rho family of GTPases. The encoded protein is localized to the outer mitochondrial membrane and plays a role in mitochondrial trafficking and fusion-fission dynamics. [provided by RefSeq, Nov 2011]

Canonical amino-acid sequenceUniProt

618 residues, UniProt reviewed canonical sequence.

>Q8IXI1|RHOT2
     1  MRRDVRILLL GEAQVGKTSL ILSLVGEEFP EEVPPRAEEI TIPADVTPEK VPTHIVDYSE
    61  AEQTDEELRE EIHKANVVCV VYDVSEEATI EKIRTKWIPL VNGGTTQGPR VPIILVGNKS
   121  DLRSGSSMEA VLPIMSQFPE IETCVECSAK NLRNISELFY YAQKAVLHPT APLYDPEAKQ
   181  LRPACAQALT RIFRLSDQDL DQALSDEELN AFQKSCFGHP LAPQALEDVK TVVCRNVAGG
   241  VREDRLTLDG FLFLNTLFIQ RGRHETTWTI LRRFGYSDAL ELTADYLSPL IHVPPGCSTE
   301  LNHLGYQFVQ RVFEKHDQDR DGALSPVELQ SLFSVFPAAP WGPELPRTVR TEAGRLPLHG
   361  YLCQWTLVTY LDVRSCLGHL GYLGYPTLCE QDQAHAITVT REKRLDQEKG QTQRSVLLCK
   421  VVGARGVGKS AFLQAFLGRG LGHQDTREQP PGYAIDTVQV NGQEKYLILC EVGTDGLLAT
   481  SLDATCDVAC LMFDGSDPKS FAHCASVYKH HYMDGQTPCL FVSSKADLPE GVAVSGPSPA
   541  EFCRKHRLPA PVPFSCAGPA EPSTTIFTQL ATMAAFPHLV HAELHPSSFW LRGLLGVVGA
   601  AVAAVLSFSL YRVLVKSQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RHOT2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.28

Highest tissue expression

67 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.43

gnomAD pLI

0

gnomAD missense Z

-1.4

DepMap mean gene effect

-0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular homeostasis
• mitochondrial outer membrane permeabilization
• mitochondrion organization
• mitochondrion transport along microtubule

Molecular functions

• ATP hydrolysis activity
• calcium ion binding
• GDP binding
• GTP binding
• GTPase activity
• magnesium ion binding

Cellular components

• membrane
• mitochondrial outer membrane
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Small GTPase
• EF-hand domain
• EF-hand domain pair
• Mitochondrial Rho GTPase 1/3, EF hand associated, type-1
• EF hand associated, type-2
• EF-Hand 1, calcium-binding site
• MIRO domain
• Mitochondrial Rho GTPase
• P-loop containing nucleoside triphosphate hydrolase
• Mitochondrial Rho GTPase/EF-hand domain-containing protein
• Ras family
• EF hand associated
• EF hand associated

KeywordsUniProt

• Calcium
• GTP-binding
• Hydrolase
• Isopeptide bond
• Magnesium
• Membrane
• Metal-binding
• Mitochondrion
• Mitochondrion outer membrane
• Nucleotide-binding
• Repeat
• Transmembrane
• Transmembrane helix
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of RHOT2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ARMCX3
• TRAK1
• AKAP1
• IPO5
• MGARP