RHOT1

Protein identityUniProt · HPA

UniProt accession

Q8IXI2

Gene

RHOT1

Ensembl

ENSG00000126858

Chromosome

17

Canonical length

618 aa

Protein class

Predicted intracellular proteins, Predicted membrane proteins

Quaternary structure

Homodimer

OverviewNCBI Gene

Predicted to enable GTP binding activity and GTPase activity. Involved in several processes, including mitochondrial outer membrane permeabilization; mitochondrion transport along microtubule; and regulation of mitochondrion organization. Located in mitochondrial outer membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

618 residues, UniProt reviewed canonical sequence.

>Q8IXI2|RHOT1
     1  MKKDVRILLV GEPRVGKTSL IMSLVSEEFP EEVPPRAEEI TIPADVTPER VPTHIVDYSE
    61  AEQSDEQLHQ EISQANVICI VYAVNNKHSI DKVTSRWIPL INERTDKDSR LPLILVGNKS
   121  DLVEYSSMET ILPIMNQYTE IETCVECSAK NLKNISELFY YAQKAVLHPT GPLYCPEEKE
   181  MKPACIKALT RIFKISDQDN DGTLNDAELN FFQRICFNTP LAPQALEDVK NVVRKHISDG
   241  VADSGLTLKG FLFLHTLFIQ RGRHETTWTV LRRFGYDDDL DLTPEYLFPL LKIPPDCTTE
   301  LNHHAYLFLQ STFDKHDLDR DCALSPDELK DLFKVFPYIP WGPDVNNTVC TNERGWITYQ
   361  GFLSQWTLTT YLDVQRCLEY LGYLGYSILT EQESQASAVT VTRDKKIDLQ KKQTQRNVFR
   421  CNVIGVKNCG KSGVLQALLG RNLMRQKKIR EDHKSYYAIN TVYVYGQEKY LLLHDISESE
   481  FLTEAEIICD VVCLVYDVSN PKSFEYCARI FKQHFMDSRI PCLIVAAKSD LHEVKQEYSI
   541  SPTDFCRKHK MPPPQAFTCN TADAPSKDIF VKLTTMAMYP HVTQADLKSS TFWLRASFGA
   601  TVFAVLGFAM YKALLKQR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RHOT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

47 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.35

gnomAD pLI

0.8

gnomAD missense Z

2.47

DepMap mean gene effect

-0.12

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular homeostasis
• mitochondrial outer membrane permeabilization
• mitochondrion organization
• mitochondrion transport along microtubule
• regulation of mitochondrion organization

Molecular functions

• ATP hydrolysis activity
• calcium ion binding
• GDP binding
• GTP binding
• GTPase activity
• magnesium ion binding

Cellular components

• membrane
• mitochondrial outer membrane
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Small GTPase
• EF-hand domain
• Small GTP-binding domain
• EF-hand domain pair
• Mitochondrial Rho GTPase 1/3, EF hand associated, type-1
• EF hand associated, type-2
• EF-Hand 1, calcium-binding site
• MIRO domain
• Mitochondrial Rho GTPase
• P-loop containing nucleoside triphosphate hydrolase
• Mitochondrial Rho GTPase/EF-hand domain-containing protein
• Ras family
• EF hand associated
• EF hand associated

KeywordsUniProt

• Acetylation
• Calcium
• GTP-binding
• Hydrolase
• Isopeptide bond
• Membrane
• Metal-binding
• Mitochondrion
• Mitochondrion outer membrane
• Nucleotide-binding
• Repeat
• Transmembrane
• Transmembrane helix
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of RHOT1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ARMCX1
• RAP1GDS1
• TRAK1
• IPO5
• PRKN