UBE2B
Ubiquitin-conjugating enzyme E2 B
Also known as: HHR6B, RAD6B, UBC2, UBE2B_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P63146
- Gene
- UBE2B
- Ensembl
- ENSG00000119048
- Chromosome
- 5
- Canonical length
- 152 aa
- Protein class
- Enzymes, Metabolic proteins, Predicted intracellular proteins
- Subcellular location
- Nucleoplasm,Vesicles,Plasma membrane,Cytosol,Flagellar centriole,Mid piece,Principal piece
OverviewNCBI Gene
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
152 residues, UniProt reviewed canonical sequence.
>P63146|UBE2B
1 MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS
61 EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS
121 PANSQAAQLY QENKREYEKR VSAIVEQSWN DSLocalizationUniProt · AlphaFold · HPA
Whether an antibody against UBE2B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Cell surface
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.28
- Highest tissue expression
- 202 nTPM
Expression across tissuesHPA
Tissue
- skeletal muscle: 202 nTPM
- tongue: 99 nTPM
- heart muscle: 84 nTPM
- blood vessel: 62 nTPM
- bone marrow: 51 nTPM
- adrenal gland: 51 nTPM
Single-cell type
- neutrophils: 1,754 nCPM
- esophageal apical cells: 1,342 nCPM
- syncytiotrophoblasts: 1,180 nCPM
- late spermatids: 476 nCPM
- pancreatic acinar cells: 425 nCPM
- myonuclei: 422 nCPM
Immune cell
- neutrophil: 313 nTPM
- basophil: 160 nTPM
- eosinophil: 123 nTPM
- non-classical monocyte: 54 nTPM
- T-reg: 52 nTPM
- naive CD4 T-cell: 50 nTPM
Brain region
- white matter: 39 nTPM
- cerebellum: 38 nTPM
- hypothalamus: 37 nTPM
- hippocampal formation: 34 nTPM
- cerebral cortex: 33 nTPM
- basal ganglia: 33 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.7
- gnomAD pLI
- 0.2
- gnomAD missense Z
- 2.18
- DepMap mean gene effect
- 0.07
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- apoptotic process
- chiasma assembly
- chromatin organization
- DNA damage response
- DNA damage tolerance
- DNA repair
- ectopic germ cell programmed cell death
- in utero embryonic development
- meiotic telomere clustering
- negative regulation of apoptotic process
- negative regulation of developmental process
- negative regulation of post-translational protein modification
- negative regulation of reproductive process
- positive regulation of canonical Wnt signaling pathway
- positive regulation of reciprocal meiotic recombination
- proteasome-mediated ubiquitin-dependent protein catabolic process
- protein autoubiquitination
- protein K11-linked ubiquitination
- protein K48-linked ubiquitination
- protein K63-linked ubiquitination
- protein polyubiquitination
- protein stabilization
- protein ubiquitination
- response to UV
- response to xenobiotic stimulus
- sperm axoneme assembly
- spermatogenesis
- ubiquitin-dependent protein catabolic process
- synaptonemal complex organization
Molecular functions
- ATP binding
- ubiquitin conjugating enzyme activity
- ubiquitin protein ligase binding
- ubiquitin-protein transferase activity
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of UBE2B in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads UBE2B as an antibody target. Whether an autoantibody or antibody against UBE2B could matter depends on whether native UBE2B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
UBE2B is annotated at the cell surface, where native UBE2B is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.
Annotation status
The present source text does not explicitly label UBE2B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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