PREX1

Protein identityUniProt · HPA

UniProt accession

Q8TCU6

Gene

PREX1

Ensembl

ENSG00000124126

Chromosome

20

Canonical length

1659 aa

Protein class

Predicted intracellular proteins

Subcellular location

Vesicles,Cytosol

OverviewNCBI Gene

The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1659 residues, UniProt reviewed canonical sequence.

>Q8TCU6|PREX1
     1  MEAPSGSEPG GDGAGDCAHP DPRAPGAAAP SSGPGPCAAA RESERQLRLR LCVLNEILGT
    61  ERDYVGTLRF LQSAFLHRIR QNVADSVEKG LTEENVKVLF SNIEDILEVH KDFLAALEYC
   121  LHPEPQSQHE LGNVFLKFKD KFCVYEEYCS NHEKALRLLV ELNKIPTVRA FLLSCMLLGG
   181  RKTTDIPLEG YLLSPIQRIC KYPLLLKELA KRTPGKHPDH PAVQSALQAM KTVCSNINET
   241  KRQMEKLEAL EQLQSHIEGW EGSNLTDICT QLLLQGTLLK ISAGNIQERA FFLFDNLLVY
   301  CKRKSRVTGS KKSTKRTKSI NGSLYIFRGR INTEVMEVEN VEDGTADYHS NGYTVTNGWK
   361  IHNTAKNKWF VCMAKTAEEK QKWLDAIIRE REQRESLKLG MERDAYVMIA EKGEKLYHMM
   421  MNKKVNLIKD RRRKLSTVPK CFLGNEFVAW LLEIGEISKT EEGVNLGQAL LENGIIHHVS
   481  DKHQFKNEQV MYRFRYDDGT YKARSELEDI MSKGVRLYCR LHSLYTPVIK DRDYHLKTYK
   541  SVLPGSKLVD WLLAQGDCQT REEAVALGVG LCNNGFMHHV LEKSEFRDES QYFRFHADEE
   601  MEGTSSKNKQ LRNDFKLVEN ILAKRLLILP QEEDYGFDIE EKNKAVVVKS VQRGSLAEVA
   661  GLQVGRKIYS INEDLVFLRP FSEVESILNQ SFCSRRPLRL LVATKAKEII KIPDQPDTLC
   721  FQIRGAAPPY VYAVGRGSEA MAAGLCAGQC ILKVNGSNVM NDGAPEVLEH FQAFRSRREE
   781  ALGLYQWIYH THEDAQEARA SQEASTEDPS GEQAQEEDQA DSAFPLLSLG PRLSLCEDSP
   841  MVTLTVDNVH LEHGVVYEYV STAGVRCHVL EKIVEPRGCF GLTAKILEAF AANDSVFVEN
   901  CRRLMALSSA IVTMPHFEFR NICDTKLESI GQRIACYQEF AAQLKSRVSP PFKQAPLEPH
   961  PLCGLDFCPT NCHINLMEVS YPKTTPSVGR SFSIRFGRKP SLIGLDPEQG HLNPMSYTQH
  1021  CITTMAAPSW KCLPAAEGDP QGQGLHDGSF GPASGTLGQE DRGLSFLLKQ EDREIQDAYL
  1081  QLFTKLDVAL KEMKQYVTQI NRLLSTITEP TSGGSCDASL AEEASSLPLV SEESEMDRSD
  1141  HGGIKKVCFK VAEEDQEDSG HDTMSYRDSY SECNSNRDSV LSYTSVRSNS SYLGSDEMGS
  1201  GDELPCDMRI PSDKQDKLHG CLEHLFNQVD SINALLKGPV MSRAFEETKH FPMNHSLQEF
  1261  KQKEECTIRG RSLIQISIQE DPWNLPNSIK TLVDNIQRYV EDGKNQLLLA LLKCTDTELQ
  1321  LRRDAIFCQA LVAAVCTFSK QLLAALGYRY NNNGEYEESS RDASRKWLEQ VAATGVLLHC
  1381  QSLLSPATVK EERTMLEDIW VTLSELDNVT FSFKQLDENY VANTNVFYHI EGSRQALKVI
  1441  FYLDSYHFSK LPSRLEGGAS LRLHTALFTK VLENVEGLPS PGSQAAEDLQ QDINAQSLEK
  1501  VQQYYRKLRA FYLERSNLPT DASTTAVKID QLIRPINALD ELCRLMKSFV HPKPGAAGSV
  1561  GAGLIPISSE LCYRLGACQM VMCGTGMQRS TLSVSLEQAA ILARSHGLLP KCIMQATDIM
  1621  RKQGPRVEIL AKNLRVKDQM PQGAPRLYRL CQPPVDGDL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PREX1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.3

Highest tissue expression

50 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.09

gnomAD pLI

1

gnomAD missense Z

3.7

DepMap mean gene effect

-0.12

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• actin filament polymerization
• G protein-coupled receptor signaling pathway
• intracellular signal transduction
• negative regulation of TOR signaling
• neutrophil activation
• neutrophil chemotaxis
• positive regulation of cell migration
• positive regulation of substrate adhesion-dependent cell spreading
• regulation of actin filament polymerization
• regulation of dendrite development
• regulation of small GTPase mediated signal transduction
• superoxide metabolic process
• T cell differentiation

Molecular functions

• enzyme binding
• GTPase activator activity
• guanyl-nucleotide exchange factor activity
• phospholipid binding
• protein serine/threonine kinase inhibitor activity

Cellular components

• cytosol
• dendritic shaft
• growth cone
• perinuclear region of cytoplasm
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Dbl homology domain
• DEP domain
• Guanine-nucleotide dissociation stimulator, CDC24, conserved site
• PDZ domain
• Pleckstrin homology domain
• PH-like domain superfamily
• Dbl homology (DH) domain superfamily
• PDZ superfamily
• Winged helix-like DNA-binding domain superfamily
• Winged helix DNA-binding domain superfamily
• Regulators of mTOR signaling and Rac activation
• SOS1/NGEF-like, PH domain
• Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP)
• RhoGEF domain
• SOS1/NGEF-like PH domain

KeywordsUniProt

• Cell membrane
• Cytoplasm
• Guanine-nucleotide releasing factor
• Membrane
• Phosphoprotein
• Repeat

InteractionsUniProt · HPA

Protein binding partners of PREX1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• AUTS2
• RAC1
• MTOR
• RICTOR
• RPTOR