Seroatlas · Human Serome Atlas

POLE3

DNA polymerase epsilon subunit 3

Also known as: CHARAC17, CHRAC17, CHRAC2, DPOE3_HUMAN, p17, Ybl1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NRF9
Gene: POLE3
Ensembl: ENSG00000148229
Chromosome: 9
Canonical length: 147 aa
Protein class: FDA approved drug targets, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli,Cytosol

OverviewNCBI Gene

POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]

Canonical amino-acid sequenceUniProt

147 residues, UniProt reviewed canonical sequence.

>Q9NRF9|POLE3
     1  MAERPEDLNL PNAVITRIIK EALPDGVNIS KEARSAISRA ASVFVLYATS CANNFAMKGK
    61  RKTLNASDVL SAMEEMEFQR FVTPLKEALE AYRREQKGKK EASEQKKKDK DKKTDSEEQD
   121  KSRDEDNDED EERLEEEEQN EEEEVDN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POLE3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.44
Highest tissue expression: 107 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 107 nTPM
skeletal muscle: 74 nTPM
tonsil: 53 nTPM
tongue: 53 nTPM
thymus: 51 nTPM
lymph node: 50 nTPM

Single-cell type

erythrocyte progenitors: 225 nCPM
oocytes: 120 nCPM
megakaryocyte progenitors: 115 nCPM
differentiating spermatogonia: 108 nCPM
esophageal basal cells: 107 nCPM
monocyte progenitors: 100 nCPM

Immune cell

total PBMC: 135 nTPM
MAIT T-cell: 104 nTPM
T-reg: 102 nTPM
eosinophil: 91 nTPM
intermediate monocyte: 87 nTPM
memory B-cell: 87 nTPM

Brain region

cerebellum: 30 nTPM
pons: 24 nTPM
cerebral cortex: 24 nTPM
white matter: 23 nTPM
spinal cord: 23 nTPM
basal ganglia: 22 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.28
gnomAD pLI: 0.03
gnomAD missense Z: 0.14
DepMap mean gene effect: -0.29
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Transcription factor CBF/NF-Y/archaeal histone domain
Histone-fold
Histone-like transcription factor (CBF/NF-Y) and archaeal histone
DNA Polymerase Epsilon Subunit

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of POLE3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POLE3 as an antibody target. Whether an autoantibody or antibody against POLE3 could matter depends on whether native POLE3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POLE3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POLE3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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