Seroatlas · Human Serome Atlas

POLE2

DNA polymerase epsilon subunit 2

Also known as: DPE2, DPOE2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P56282
Gene: POLE2
Ensembl: ENSG00000100479
Chromosome: 14
Canonical length: 527 aa
Protein class: FDA approved drug targets, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear bodies,Vesicles

OverviewNCBI Gene

DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency. [provided by RefSeq, Jan 2017]

Canonical amino-acid sequenceUniProt

527 residues, UniProt reviewed canonical sequence.

>P56282|POLE2
     1  MAPERLRSRA LSAFKLRGLL LRGEAIKYLT EALQSISELE LEDKLEKIIN AVEKQPLSSN
    61  MIERSVVEAA VQECSQSVDE TIEHVFNIIG AFDIPRFVYN SERKKFLPLL MTNHPAPNLF
   121  GTPRDKAEMF RERYTILHQR THRHELFTPP VIGSHPDESG SKFQLKTIET LLGSTTKIGD
   181  AIVLGMITQL KEGKFFLEDP TGTVQLDLSK AQFHSGLYTE ACFVLAEGWF EDQVFHVNAF
   241  GFPPTEPSST TRAYYGNINF FGGPSNTSVK TSAKLKQLEE ENKDAMFVFL SDVWLDQVEV
   301  LEKLRIMFAG YSPAPPTCFI LCGNFSSAPY GKNQVQALKD SLKTLADIIC EYPDIHQSSR
   361  FVFVPGPEDP GFGSILPRPP LAESITNEFR QRVPFSVFTT NPCRIQYCTQ EITVFREDLV
   421  NKMCRNCVRF PSSNLAIPNH FVKTILSQGH LTPLPLYVCP VYWAYDYALR VYPVPDLLVI
   481  ADKYDPFTTT NTECLCINPG SFPRSGFSFK VFYPSNKTVE DSKLQGF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POLE2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.24
Highest tissue expression: 17 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 17 nTPM
tonsil: 5.8 nTPM
thymus: 5.3 nTPM
lymph node: 4.9 nTPM
appendix: 4.8 nTPM
rectum: 4.5 nTPM

Single-cell type

erythrocyte progenitors: 181 nCPM
megakaryocyte progenitors: 135 nCPM
megakaryocyte-erythroid progenitors: 84 nCPM
differentiating spermatogonia: 82 nCPM
oocytes: 55 nCPM
cardiomyocytes: 52 nCPM

Immune cell

NK-cell: 1.1 nTPM
T-reg: 0.7 nTPM
memory CD8 T-cell: 0.5 nTPM
memory B-cell: 0.3 nTPM
naive B-cell: 0.3 nTPM
eosinophil: 0.2 nTPM

Brain region

cerebellum: 3.7 nTPM
white matter: 3.7 nTPM
cerebral cortex: 3.6 nTPM
midbrain: 3.6 nTPM
basal ganglia: 3.5 nTPM
medulla oblongata: 3.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.76
gnomAD pLI: 0
gnomAD missense Z: 0.79
DepMap mean gene effect: -1.61
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

DNA polymerase alpha/delta/epsilon, subunit B
DNA polymerase alpha/epsilon subunit B
DNA polymerase epsilon, subunit B
DNA polymerase epsilon subunit B, N-terminal
DNA polymerases epsilon N terminal

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of POLE2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POLE2 as an antibody target. Whether an autoantibody or antibody against POLE2 could matter depends on whether native POLE2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POLE2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POLE2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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