Seroatlas · Human Serome Atlas

PKMYT1

Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase

Also known as: MYT1, PMYT1_HUMAN, PPP1R126

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q99640
Gene
PKMYT1
Ensembl
ENSG00000127564
Chromosome
16
Canonical length
499 aa
Protein class
Enzymes, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location
Nucleoplasm,Nucleoli,Golgi apparatus

OverviewNCBI Gene

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Canonical amino-acid sequenceUniProt

499 residues, UniProt reviewed canonical sequence.

>Q99640|PKMYT1
     1  MLERPPALAM PMPTEGTPPP LSGTPIPVPA YFRHAEPGFS LKRPRGLSRS LPPPPPAKGS
    61  IPISRLFPPR TPGWHQLQPR RVSFRGEASE TLQSPGYDPS RPESFFQQSF QRLSRLGHGS
   121  YGEVFKVRSK EDGRLYAVKR SMSPFRGPKD RARKLAEVGS HEKVGQHPCC VRLEQAWEEG
   181  GILYLQTELC GPSLQQHCEA WGASLPEAQV WGYLRDTLLA LAHLHSQGLV HLDVKPANIF
   241  LGPRGRCKLG DFGLLVELGT AGAGEVQEGD PRYMAPELLQ GSYGTAADVF SLGLTILEVA
   301  CNMELPHGGE GWQQLRQGYL PPEFTAGLSS ELRSVLVMML EPDPKLRATA EALLALPVLR
   361  QPRAWGVLWC MAAEALSRGW ALWQALLALL CWLWHGLAHP ASWLQPLGPP ATPPGSPPCS
   421  LLLDSSLSSN WDDDSLGPSL SPEAVLARTV GSTSTPRSRC TPRDALDLSD INSEPPRGSF
   481  PSFEPRNLLS LFEDTLDPT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PKMYT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.44
Highest tissue expression
58 nTPM

Expression across tissuesHPA

Tissue

  • testis: 58 nTPM
  • bone marrow: 26 nTPM
  • retina: 16 nTPM
  • esophagus: 12 nTPM
  • thymus: 8.9 nTPM
  • lymph node: 7.5 nTPM

Single-cell type

  • early primary spermatocytes: 126 nCPM
  • late primary spermatocytes: 70 nCPM
  • extravillous trophoblasts: 63 nCPM
  • erythrocyte progenitors: 40 nCPM
  • migrating cytotrophoblasts: 33 nCPM
  • differentiating spermatogonia: 33 nCPM

Immune cell

  • T-reg: 1.3 nTPM
  • total PBMC: 0.3 nTPM
  • memory B-cell: 0.2 nTPM
  • classical monocyte: 0.1 nTPM
  • eosinophil: 0.1 nTPM
  • intermediate monocyte: 0.1 nTPM

Brain region

  • cerebellum: 4 nTPM
  • pons: 2.2 nTPM
  • thalamus: 2.1 nTPM
  • white matter: 2.1 nTPM
  • cerebral cortex: 2 nTPM
  • medulla oblongata: 2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.52
gnomAD pLI
0.22
gnomAD missense Z
0.07
DepMap mean gene effect
-1.24
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PKMYT1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PKMYT1 as an antibody target. Whether an autoantibody or antibody against PKMYT1 could matter depends on whether native PKMYT1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PKMYT1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PKMYT1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PKMYT1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...