PFKFB2

Protein identityUniProt · HPA

UniProt accession

O60825

Gene

PFKFB2

Ensembl

ENSG00000123836

Chromosome

1

Canonical length

505 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm

Quaternary structure

Homodimer

OverviewNCBI Gene

The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

505 residues, UniProt reviewed canonical sequence.

>O60825|PFKFB2
     1  MSGASSSEQN NNSYETKTPN LRMSEKKCSW ASYMTNSPTL IVMIGLPARG KTYVSKKLTR
    61  YLNWIGVPTK VFNLGVYRRE AVKSYKSYDF FRHDNEEAMK IRKQCALVAL EDVKAYLTEE
   121  NGQIAVFDAT NTTRERRDMI LNFAEQNSFK VFFVESVCDD PDVIAANILE VKVSSPDYPE
   181  RNRENVMEDF LKRIECYKVT YRPLDPDNYD KDLSFIKVIN VGQRFLVNRV QDYIQSKIVY
   241  YLMNIHVQPR TIYLCRHGES EFNLLGKIGG DSGLSVRGKQ FAQALRKFLE EQEITDLKVW
   301  TSQLKRTIQT AESLGVPYEQ WKILNEIDAG VCEEMTYAEI EKRYPEEFAL RDQEKYLYRY
   361  PGGESYQDLV QRLEPVIMEL ERQGNVLVIS HQAVMRCLLA YFLDKGADEL PYLRCPLHTI
   421  FKLTPVAYGC KVETIKLNVE AVNTHRDKPT NNFPKNQTPV RMRRNSFTPL SSSNTIRRPR
   481  NYSVGSRPLK PLSPLRAQDM QEGAD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PFKFB2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.31

Highest tissue expression

77 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.78

gnomAD pLI

0

gnomAD missense Z

2.45

DepMap mean gene effect

-0.07

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• fructose 2,6-bisphosphate metabolic process
• fructose metabolic process
• gluconeogenesis
• glucose catabolic process
• glycolytic process
• lactate biosynthetic process
• positive regulation of insulin secretion
• pyruvate biosynthetic process
• response to glucose

Molecular functions

• 6-phosphofructo-2-kinase activity
• ATP binding
• fructose-2,6-bisphosphate 2-phosphatase activity
• protein kinase binding

Cellular components

• 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex
• cytosol
• nucleoplasm

Protein domainsUniProt · Pfam · InterPro

• Phosphoglycerate/bisphosphoglycerate mutase, active site
• Fructose-2,6-bisphosphatase
• Histidine phosphatase superfamily, clade-1
• 6-phosphofructo-2-kinase
• P-loop containing nucleoside triphosphate hydrolase
• Histidine phosphatase superfamily
• Histidine phosphatase superfamily (branch 1)
• 6-phosphofructo-2-kinase

KeywordsUniProt

• Acetylation
• ATP-binding
• Hydrolase
• Kinase
• Multifunctional enzyme
• Nucleotide-binding
• Phosphoprotein
• Transferase

InteractionsUniProt · HPA

Protein binding partners of PFKFB2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• YWHAB
• YWHAE
• YWHAG
• YWHAH
• YWHAQ
• YWHAZ