NXF3

Protein identityUniProt · HPA

UniProt accession

Q9H4D5

Gene

NXF3

Ensembl

ENSG00000147206

Chromosome

X

Canonical length

531 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

531 residues, UniProt reviewed canonical sequence.

>Q9H4D5|NXF3
     1  MSLPSGHTTG HTDQVVQRRA RCWDIYQRRF SSRSEPVNPG MHSSSHQQQD GDAAMHGAHM
    61  DSPVRYTPYT ISPYNRKGSF RKQDQTHVNM EREQKPPERR MEGNMPDGTL GSWFKITVPF
   121  GIKYNEKWLL NLIQNECSVP FVPVEFHYEN MHASFFVENA SIAYALKNVS GKIWDEDNEK
   181  ISIFVNPAGI PHFVHRELKS EKVEQIKLAM NQQCDVSQEA LDIQRLPFYP DMVNRDTKMA
   241  SNPRKCMAAS LDVHEENIPT VMSAGEMDKW KGIEPGEKCA DRSPVCTTFS DTSSNINSIL
   301  ELFPKLLCLD GQQSPRATLC GTEAHKRLPT CKGSFFGSEM LKNLVLQFLQ QYYLIYDSGD
   361  RQGLLSAYHD EACFSLSIPF NPEDSAPSSF CKFFKDSRNI KILKDPYLRG ELLKHTKLDI
   421  VDSLSALPKT QHDLSSFLVD MWYQTEWMLC FSVNGVFKEV EGQSQGSVLA FTRTFIATPG
   481  SSSSLCIVND KLFVRDTSHQ GTQSALFTLV PTAFSSSVPA FSQEQQKMLP S

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NXF3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.49

Highest tissue expression

57 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NXF3.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 90 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Male infertility

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.6

gnomAD pLI

0.01

gnomAD missense Z

0.77

DepMap mean gene effect

0.05

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• mRNA export from nucleus
• poly(A)+ mRNA export from nucleus

Molecular functions

• RNA binding

Cellular components

• cytoplasm
• nuclear RNA export factor complex
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Nuclear transport factor 2 domain
• Nucleotide-binding alpha-beta plait domain superfamily
• Nuclear RNA export factor Tap, RNA-binding domain
• Nuclear transport factor 2, eukaryote
• Nuclear RNA export factor
• Leucine-rich repeat domain superfamily
• NTF2-like domain superfamily
• RNA-binding domain superfamily
• NXF1/2/3/5-like, leucine-rich repeat domain
• Tap, RNA-binding
• Nuclear RNA export factor, NTF2 domain
• NXF1/2/3/5 leucine-rich repeat domain

KeywordsUniProt

• Cytoplasm
• mRNA transport
• Nucleus
• RNA-binding
• Transport

InteractionsUniProt · HPA

Protein binding partners of NXF3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• E1B-AP5
• NXT1
• NXT2