Seroatlas · Human Serome Atlas

NCF2

Neutrophil cytosol factor 2

Also known as: NCF2_HUMAN, NOXA2, p67phox

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P19878
Gene
NCF2
Ensembl
ENSG00000116701
Chromosome
1
Canonical length
526 aa
Protein class
Disease related genes, Human disease related genes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Cytosol

OverviewNCBI Gene

This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

Canonical amino-acid sequenceUniProt

526 residues, UniProt reviewed canonical sequence.

>P19878|NCF2
     1  MSLVEAISLW NEGVLAADKK DWKGALDAFS AVQDPHSRIC FNIGCMYTIL KNMTEAEKAF
    61  TRSINRDKHL AVAYFQRGML YYQTEKYDLA IKDLKEALIQ LRGNQLIDYK ILGLQFKLFA
   121  CEVLYNIAFM YAKKEEWKKA EEQLALATSM KSEPRHSKID KAMECVWKQK LYEPVVIPVG
   181  KLFRPNERQV AQLAKKDYLG KATVVASVVD QDSFSGFAPL QPQAAEPPPR PKTPEIFRAL
   241  EGEAHRVLFG FVPETKEELQ VMPGNIVFVL KKGNDNWATV MFNGQKGLVP CNYLEPVELR
   301  IHPQQQPQEE SSPQSDIPAP PSSKAPGRPQ LSPGQKQKEE PKEVKLSVPM PYTLKVHYKY
   361  TVVMKTQPGL PYSQVRDMVS KKLELRLEHT KLSYRPRDSN ELVPLSEDSM KDAWGQVKNY
   421  CLTLWCENTV GDQGFPDEPK ESEKADANNQ TTEPQLKKGS QVEALFSYEA TQPEDLEFQE
   481  GDIILVLSKV NEEWLEGECK GKVGIFPKVF VEDCATTDLE STRREV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NCF2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.4
Highest tissue expression
119 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 119 nTPM
  • spleen: 74 nTPM
  • lung: 61 nTPM
  • appendix: 59 nTPM
  • placenta: 30 nTPM
  • adipose tissue: 21 nTPM

Single-cell type

  • neutrophils: 1,819 nCPM
  • neutrophil progenitors: 434 nCPM
  • monocytes: 348 nCPM
  • hofbauer cells: 204 nCPM
  • monocyte progenitors: 183 nCPM
  • cdc: 138 nCPM

Immune cell

  • eosinophil: 1,254 nTPM
  • neutrophil: 745 nTPM
  • classical monocyte: 473 nTPM
  • intermediate monocyte: 339 nTPM
  • non-classical monocyte: 318 nTPM
  • total PBMC: 286 nTPM

Brain region

  • thalamus: 15 nTPM
  • cerebral cortex: 15 nTPM
  • white matter: 10 nTPM
  • pons: 6.7 nTPM
  • medulla oblongata: 6.2 nTPM
  • spinal cord: 5 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NCF2.

Disease | AllUniProt

Conditions NCF2 is implicated in, by any mechanism.

  • Granulomatous disease, chronic, autosomal recessive, 2 (CGD2) MIM:233710

Disease | GeneticClinVar

72 pathogenic / likely-pathogenic of 675 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.74
gnomAD pLI
0
gnomAD missense Z
1.04
DepMap mean gene effect
0.09
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NCF2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NCF2 as an antibody target. Whether an autoantibody or antibody against NCF2 could matter depends on whether native NCF2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NCF2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NCF2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NCF2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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