Seroatlas · Human Serome Atlas

MARCHF2

E3 ubiquitin-protein ligase MARCHF2

Also known as: HSPC240, MARCH-II, MARCH2, MARH2_HUMAN, RNF172

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9P0N8
Gene: MARCHF2
Ensembl: ENSG00000099785
Chromosome: 19
Canonical length: 246 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins, Transporters
Subcellular location: Endoplasmic reticulum,Cytosol

OverviewNCBI Gene

MARCH2 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH2 reduces surface accumulation of several glycoproteins and appears to regulate early endosome-to-trans-Golgi network (TGN) trafficking (Bartee et al., 2004 [PubMed 14722266]; Nakamura et al., 2005 [PubMed 15689499]).[supplied by OMIM, Mar 2010]

Canonical amino-acid sequenceUniProt

246 residues, UniProt reviewed canonical sequence.

>Q9P0N8|MARCHF2
     1  MTTGDCCHLP GSLCDCSGSP AFSKVVEATG LGPPQYVAQV TSRDGRLLST VIRALDTPSD
    61  GPFCRICHEG ANGECLLSPC GCTGTLGAVH KSCLEKWLSS SNTSYCELCH TEFAVEKRPR
   121  PLTEWLKDPG PRTEKRTLCC DMVCFLFITP LAAISGWLCL RGAQDHLRLH SQLEAVGLIA
   181  LTIALFTIYV LWTLVSFRYH CQLYSEWRKT NQKVRLKIRE ADSPEGPQHS PLAAGLLKKV
   241  AEETPV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MARCHF2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 2
Mean surface accessibility (rSASA): 0.49
Highest tissue expression: 99 nTPM

Expression across tissuesHPA

Tissue

amygdala: 99 nTPM
heart muscle: 97 nTPM
spinal cord: 93 nTPM
colon: 93 nTPM
cerebral cortex: 93 nTPM
midbrain: 90 nTPM

Single-cell type

platelets: 1,775 nCPM
megakaryocytes: 507 nCPM
epididymal principal cells: 158 nCPM
kupffer cells: 120 nCPM
hofbauer cells: 110 nCPM
pdcs: 90 nCPM

Immune cell

basophil: 53 nTPM
plasmacytoid DC: 52 nTPM
eosinophil: 31 nTPM
intermediate monocyte: 29 nTPM
non-classical monocyte: 26 nTPM
neutrophil: 23 nTPM

Brain region

medulla oblongata: 146 nTPM
spinal cord: 137 nTPM
pons: 131 nTPM
thalamus: 130 nTPM
midbrain: 121 nTPM
amygdala: 120 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.89
gnomAD pLI: 0.03
DepMap mean gene effect: -0.04
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MARCHF2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MARCHF2 as an antibody target. Whether an autoantibody or antibody against MARCHF2 could matter depends on whether native MARCHF2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MARCHF2 is annotated at the cell surface, where native MARCHF2 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label MARCHF2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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