Seroatlas · Human Serome Atlas

MARCHF3

E3 ubiquitin-protein ligase MARCHF3

Also known as: MARCH-III, MARCH3, MARH3_HUMAN, MGC48332, RNF173

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q86UD3
Gene: MARCHF3
Ensembl: ENSG00000173926
Chromosome: 5
Canonical length: 253 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins, Transporters
Subcellular location: Vesicles

OverviewNCBI Gene

This gene encodes a member of the membrane-associated RING-CH (MARCH) family. The encoded protein is an E3 ubiquitin-protein ligase that may be involved in regulation of the endosomal transport pathway. [provided by RefSeq, Mar 2013]

Canonical amino-acid sequenceUniProt

253 residues, UniProt reviewed canonical sequence.

>Q86UD3|MARCHF3
     1  MTTSRCSHLP EVLPDCTSSA APVVKTVEDC GSLVNGQPQY VMQVSAKDGQ LLSTVVRTLA
    61  TQSPFNDRPM CRICHEGSSQ EDLLSPCECT GTLGTIHRSC LEHWLSSSNT SYCELCHFRF
   121  AVERKPRPLV EWLRNPGPQH EKRTLFGDMV CFLFITPLAT ISGWLCLRGA VDHLHFSSRL
   181  EAVGLIALTV ALFTIYLFWT LVSFRYHCRL YNEWRRTNQR VILLIPKSVN VPSNQPSLLG
   241  LHSVKRNSKE TVV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MARCHF3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 2
Mean surface accessibility (rSASA): 0.46
Highest tissue expression: 8 nTPM

Expression across tissuesHPA

Tissue

heart muscle: 8 nTPM
urinary bladder: 7 nTPM
retina: 6.9 nTPM
skin: 6.8 nTPM
skeletal muscle: 6.1 nTPM
adipose tissue: 6 nTPM

Single-cell type

mast cells: 896 nCPM
neutrophils: 550 nCPM
retinal horizontal cells: 483 nCPM
microglia: 462 nCPM
colonocytes: 402 nCPM
paneth cells: 344 nCPM

Immune cell

naive B-cell: 12 nTPM
memory B-cell: 11 nTPM
T-reg: 2.9 nTPM
eosinophil: 2.8 nTPM
naive CD4 T-cell: 2.3 nTPM
NK-cell: 2.3 nTPM

Brain region

medulla oblongata: 29 nTPM
spinal cord: 22 nTPM
white matter: 22 nTPM
thalamus: 21 nTPM
pons: 21 nTPM
midbrain: 20 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.73
gnomAD pLI: 0.17
DepMap mean gene effect: -0.08
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MARCHF3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MARCHF3 as an antibody target. Whether an autoantibody or antibody against MARCHF3 could matter depends on whether native MARCHF3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MARCHF3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MARCHF3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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