MAPKAPK3

Protein identityUniProt · HPA

UniProt accession

Q16644

Gene

MAPKAPK3

Ensembl

ENSG00000114738

Chromosome

3

Canonical length

382 aa

Protein class

Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Canonical amino-acid sequenceUniProt

382 residues, UniProt reviewed canonical sequence.

>Q16644|MAPKAPK3
     1  MDGETAEEQG GPVPPPVAPG GPGLGGAPGG RREPKKYAVT DDYQLSKQVL GLGVNGKVLE
    61  CFHRRTGQKC ALKLLYDSPK ARQEVDHHWQ ASGGPHIVCI LDVYENMHHG KRCLLIIMEC
   121  MEGGELFSRI QERGDQAFTE REAAEIMRDI GTAIQFLHSH NIAHRDVKPE NLLYTSKEKD
   181  AVLKLTDFGF AKETTQNALQ TPCYTPYYVA PEVLGPEKYD KSCDMWSLGV IMYILLCGFP
   241  PFYSNTGQAI SPGMKRRIRL GQYGFPNPEW SEVSEDAKQL IRLLLKTDPT ERLTITQFMN
   301  HPWINQSMVV PQTPLHTARV LQEDKDHWDE VKEEMTSALA TMRVDYDQVK IKDLKTSNNR
   361  LLNKRRKKQA GSSSASQGCN NQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAPKAPK3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.33

Highest tissue expression

265 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MAPKAPK3.

Disease | AllUniProt

Conditions MAPKAPK3 is implicated in, by any mechanism.

• Macular dystrophy, patterned, 3 (MDPT3) MIM:617111

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 320 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Patterned macular dystrophy 3

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.12

gnomAD pLI

0

gnomAD missense Z

1.44

DepMap mean gene effect

0.04

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• intracellular signal transduction
• macropinocytosis
• MAPK cascade
• response to cytokine
• response to lipopolysaccharide
• signal transduction
• toll-like receptor signaling pathway
• vascular endothelial growth factor receptor signaling pathway

Molecular functions

• ATP binding
• calcium-dependent protein serine/threonine kinase activity
• calcium/calmodulin-dependent protein kinase activity
• calmodulin binding
• MAP kinase kinase activity
• mitogen-activated protein kinase binding
• protein serine kinase activity
• protein serine/threonine kinase activity

Cellular components

• cytoplasm
• cytosol
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Protein kinase domain
• Serine/threonine-protein kinase, active site
• Protein kinase-like domain superfamily
• Protein kinase, ATP binding site
• MAP kinase activated protein kinase, C-terminal
• Protein kinase domain

KeywordsUniProt

• Acetylation
• ATP-binding
• Cytoplasm
• Kinase
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• Serine/threonine-protein kinase
• Transferase

InteractionsUniProt · HPA

Protein binding partners of MAPKAPK3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TCF3
• HSPB1
• MAPK11
• MAPK14
• MAPKAPK2