MAP2K4

Protein identityUniProt · HPA

UniProt accession

P45985

Gene

MAP2K4

Ensembl

ENSG00000065559

Chromosome

17

Canonical length

399 aa

Protein class

Cancer-related genes, Enzymes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Cell Junctions

OverviewNCBI Gene

This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Canonical amino-acid sequenceUniProt

399 residues, UniProt reviewed canonical sequence.

>P45985|MAP2K4
     1  MAAPSPSGGG GSGGGSGSGT PGPVGSPAPG HPAVSSMQGK RKALKLNFAN PPFKSTARFT
    61  LNPNPTGVQN PHIERLRTHS IESSGKLKIS PEQHWDFTAE DLKDLGEIGR GAYGSVNKMV
   121  HKPSGQIMAV KRIRSTVDEK EQKQLLMDLD VVMRSSDCPY IVQFYGALFR EGDCWICMEL
   181  MSTSFDKFYK YVYSVLDDVI PEEILGKITL ATVKALNHLK ENLKIIHRDI KPSNILLDRS
   241  GNIKLCDFGI SGQLVDSIAK TRDAGCRPYM APERIDPSAS RQGYDVRSDV WSLGITLYEL
   301  ATGRFPYPKW NSVFDQLTQV VKGDPPQLSN SEEREFSPSF INFVNLCLTK DESKRPKYKE
   361  LLKHPFILMY EERAVEVACY VCKILDQMPA TPSSPMYVD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAP2K4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.36

Highest tissue expression

42 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.22

gnomAD pLI

1

gnomAD missense Z

3.23

DepMap mean gene effect

0.08

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to mechanical stimulus
• cellular senescence
• Fc-epsilon receptor signaling pathway
• intrinsic apoptotic signaling pathway in response to hydrogen peroxide
• JNK cascade
• MAPK cascade
• negative regulation of motor neuron apoptotic process
• response to wounding
• signal transduction
• smooth muscle cell apoptotic process

Molecular functions

• ATP binding
• JUN kinase kinase activity
• MAP kinase kinase activity
• molecular adaptor activity
• protein kinase activity
• protein serine kinase activity
• protein serine/threonine kinase activity
• protein tyrosine kinase activity

Cellular components

• cytosol
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Protein kinase domain
• Serine/threonine-protein kinase, active site
• Protein kinase-like domain superfamily
• Protein kinase, ATP binding site
• Protein kinase domain

KeywordsUniProt

• Acetylation
• Apoptosis
• ATP-binding
• Cytoplasm
• Kinase
• Methylation
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• Serine/threonine-protein kinase
• Stress response
• Transferase
• Tyrosine-protein kinase

InteractionsUniProt · HPA

Protein binding partners of MAP2K4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ARRB1
• ARRB2
• SPAG9
• MAP3K1
• MAPK8
• MAPK9