KIF14

Protein identityUniProt · HPA

UniProt accession

Q15058

Gene

KIF14

Ensembl

ENSG00000118193

Chromosome

1

Canonical length

1648 aa

Protein class

Disease related genes, Human disease related genes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Plasma membrane,Midbody ring,Cytosol,Calyx,Connecting piece,Mid piece,Principal piece,End piece

OverviewNCBI Gene

This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]

Canonical amino-acid sequenceUniProt

1648 residues, UniProt reviewed canonical sequence.

>Q15058|KIF14
     1  MSLHSTHNRN NSGDILDIPS SQNSSSLNAL THSSRLKLHL KSDMSECEND DPLLRSAGKV
    61  RDINRTYVIS ASRKTADMPL TPNPVGRLAL QRRTTRNKES SLLVSELEDT TEKTAETRLT
   121  LQRRAKTDSA EKWKTAEIDS VKMTLNVGGE TENNGVSKES RTNVRIVNNA KNSFVASSVP
   181  LDEDPQVIEM MADKKYKETF SAPSRANENV ALKYSSNRPP IASLSQTEVV RSGHLTTKPT
   241  QSKLDIKVLG TGNLYHRSIG KEIAKTSNKF GSLEKRTPTK CTTEHKLTTK CSLPQLKSPA
   301  PSILKNRMSN LQVKQRPKSS FLANKQERSA ENTILPEEET VVQNTSAGKD PLKVENSQVT
   361  VAVRVRPFTK REKIEKASQV VFMSGKEITV EHPDTKQVYN FIYDVSFWSF DECHPHYASQ
   421  TTVYEKLAAP LLERAFEGFN TCLFAYGQTG SGKSYTMMGF SEEPGIIPRF CEDLFSQVAR
   481  KQTQEVSYHI EMSFFEVYNE KIHDLLVCKD ENGQRKQPLR VREHPVYGPY VEALSMNIVS
   541  SYADIQSWLE LGNKQRATAA TGMNDKSSRS HSVFTLVMTQ TKTEFVEGEE HDHRITSRIN
   601  LIDLAGSERC STAHTNGDRL KEGVSINKSL LTLGKVISAL SEQANQRSVF IPYRESVLTW
   661  LLKESLGGNS KTAMIATISP AASNIEETLS TLRYANQARL IVNIAKVNED MNAKLIRELK
   721  AEIAKLKAAQ RNSRNIDPER YRLCRQEITS LRMKLHQQER DMAEMQRVWK EKFEQAEKRK
   781  LQETKELQKA GIMFQMDNHL PNLVNLNEDP QLSEMLLYMI KEGTTTVGKY KPNSSHDIQL
   841  SGVLIADDHC TIKNFGGTVS IIPVGEAKTY VNGKHILEIT VLRHGDRVIL GGDHYFRFNH
   901  PVEVQKGKRP SGRDTPISEG PKDFEFAKNE LLMAQRSQLE AEIKEAQLKA KEEMMQGIQI
   961  AKEMAQQELS SQKAAYESKI KALEAELREE SQRKKMQEIN NQKANHKIEE LEKAKQHLEQ
  1021  EIYVNKKRLE METLATKQAL EDHSIRHARI LEALETEKQK IAKEVQILQQ NRNNRDKTFT
  1081  VQTTWSSMKL SMMIQEANAI SSKLKTYYVF GRHDISDKSS SDTSIRVRNL KLGISTFWSL
  1141  EKFESKLAAM KELYESNGSN RGEDAFCDPE DEWEPDITDA PVSSLSRRRS RSLMKNRRIS
  1201  GCLHDIQVHP IKNLHSSHSS GLMDKSSTIY SNSAESFLPG ICKELIGSSL DFFGQSYDEE
  1261  RTIADSLINS FLKIYNGLFA ISKAHEEQDE ESQDNLFSSD RAIQSLTIQT ACAFEQLVVL
  1321  MKHWLSDLLP CTNIARLEDE LRQEVKKLGG YLQLFLQGCC LDISSMIKEA QKNAIQIVQQ
  1381  AVKYVGQLAV LKGSKLHFLE NGNNKAASVQ EEFMDAVCDG VGLGMKILLD SGLEKAKELQ
  1441  HELFRQCTKN EVTKEMKTNA MGLIRSLENI FAESKIKSFR RQVQEENFEY QDFKRMVNRA
  1501  PEFLKLKHCL EKAIEIIISA LKGCHSDINL LQTCVESIRN LASDFYSDFS VPSTSVGSYE
  1561  SRVTHIVHQE LESLAKSLLF CFESEESPDL LKPWETYNQN TKEEHQQSKS SGIDGSKNKG
  1621  VPKRVYELHG SSPAVSSEEC TPSRIQWV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against KIF14 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.43

Highest tissue expression

10 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about KIF14.

Disease | AllUniProt

Conditions KIF14 is implicated in, by any mechanism.

• Meckel syndrome 12 (MKS12) MIM:616258
• Microcephaly 20, primary, autosomal recessive (MCPH20) MIM:617914

Disease | GeneticClinVar

43 pathogenic / likely-pathogenic of 523 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Microcephaly 20, primary, autosomal recessive
• Lethal fetal cerebrorenogenitourinary agenesis/hypoplasia syndrome
• Joubert syndrome and related disorders
• KIF14-related disorder
• Clear cell carcinoma of kidney

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.43

gnomAD pLI

0

gnomAD missense Z

0.77

DepMap mean gene effect

-0.45

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• activation of protein kinase activity
• cell division
• cell proliferation in forebrain
• cerebellar cortex development
• cerebellar Purkinje cell layer structural organization
• cerebral cortex development
• establishment of protein localization
• hippocampus development
• microtubule-based movement
• mitotic metaphase chromosome alignment
• negative regulation of apoptotic process
• negative regulation of integrin activation
• negative regulation of neuron apoptotic process
• olfactory bulb development
• positive regulation of cell population proliferation
• positive regulation of cytokinesis
• proteasome-mediated ubiquitin-dependent protein catabolic process
• regulation of cell adhesion
• regulation of cell growth
• regulation of cell migration
• regulation of G1/S transition of mitotic cell cycle
• regulation of G2/M transition of mitotic cell cycle
• regulation of myelination
• regulation of neuron apoptotic process
• regulation of Rap protein signal transduction
• SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
• substrate adhesion-dependent cell spreading
• cerebellar granular layer structural organization
• regulation of cell maturation

Molecular functions

• ATP binding
• ATP hydrolysis activity
• microtubule binding
• microtubule motor activity
• PDZ domain binding
• plus-end-directed microtubule motor activity
• protein kinase binding
• tubulin binding

Cellular components

• cytoplasm
• cytosol
• Flemming body
• kinesin complex
• membrane
• microtubule
• midbody
• nucleus
• spindle midzone

Protein domainsUniProt · Pfam · InterPro

• Forkhead-associated (FHA) domain
• Kinesin motor domain
• SMAD/FHA domain superfamily
• Kinesin motor domain, conserved site
• P-loop containing nucleoside triphosphate hydrolase
• Kinesin-associated
• Kinesin motor domain superfamily
• Kinesin motor domain
• FHA domain
• Kinesin-associated
• KIF14, four-helical bundle
• KIF14 four-helical bundle

KeywordsUniProt

• ATP-binding
• Ciliopathy
• Coiled coil
• Cytoplasm
• Cytoskeleton
• Meckel syndrome
• Microtubule
• Motor protein
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• Primary microcephaly

InteractionsUniProt · HPA

Protein binding partners of KIF14 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• AKT1
• ARRB2
• KIF20A
• KIF23
• RADIL
• SVIL
• PRC1
• GORASP2
• KIFBP
• TJP2