H1-2

Protein identityUniProt · HPA

UniProt accession

P16403

Gene

H1-2

Ensembl

ENSG00000187837

Chromosome

6

Canonical length

213 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nucleoli rim

OverviewNCBI Gene

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

Canonical amino-acid sequenceUniProt

213 residues, UniProt reviewed canonical sequence.

>P16403|H1-2
     1  MSETAPAAPA AAPPAEKAPV KKKAAKKAGG TPRKASGPPV SELITKAVAA SKERSGVSLA
    61  ALKKALAAAG YDVEKNNSRI KLGLKSLVSK GTLVQTKGTG ASGSFKLNKK AASGEAKPKV
   121  KKAGGTKPKK PVGAAKKPKK AAGGATPKKS AKKTPKKAKK PAAATVTKKV AKSPKKAKVA
   181  KPKKAAKSAA KAVKPKAAKP KVVKPKKAAP KKK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against H1-2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.61

Highest tissue expression

271 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about H1-2.

Disease | ImmuneIEDB

Conditions an epitope on H1-2 was assayed in.

• systemic scleroderma B cell
• systemic lupus erythematosus B cell
• Sjogren's syndrome B cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.86

gnomAD pLI

0.03

DepMap mean gene effect

-0.38

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• chromosome condensation
• facultative heterochromatin formation
• negative regulation of DNA recombination
• negative regulation of transcription by RNA polymerase II
• nucleosome assembly
• regulation of mRNA splicing, via spliceosome

Molecular functions

• chromatin DNA binding
• double-stranded DNA binding
• histone H3K27me3 reader activity
• nucleosomal DNA binding
• RNA binding
• structural constituent of chromatin

Cellular components

• euchromatin
• heterochromatin
• nucleolus
• nucleosome
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Linker histone H1/H5, domain H15
• Linker histone H1/H5
• Winged helix-like DNA-binding domain superfamily
• Winged helix DNA-binding domain superfamily
• linker histone H1 and H5 family

KeywordsUniProt

• Acetylation
• ADP-ribosylation
• Chromosome
• Citrullination
• DNA-binding
• Hydroxylation
• Methylation
• Nucleus
• Phosphoprotein

InteractionsUniProt · HPA

Protein binding partners of H1-2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• RNF146
• BAZ1B
• DDX21
• FTSJ3
• H2AC21
• H2BC21
• HMGA1
• NUCKS1