Seroatlas · Human Serome Atlas

NUCKS1

Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1

Also known as: NUCKS, NUCKS_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9H1E3
Gene
NUCKS1
Ensembl
ENSG00000069275
Chromosome
1
Canonical length
243 aa
Protein class
Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nucleoli

OverviewNCBI Gene

This gene encodes a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated in vivo by Cdk1 during mitosis of the cell cycle. [provided by RefSeq, Aug 2010]

Canonical amino-acid sequenceUniProt

243 residues, UniProt reviewed canonical sequence.

>Q9H1E3|NUCKS1
     1  MSRPVRNRKV VDYSQFQESD DADEDYGRDS GPPTKKIRSS PREAKNKRRS GKNSQEDSED
    61  SEDKDVKTKK DDSHSAEDSE DEKEDHKNVR QQRQAASKAA SKQREMLMED VGSEEEQEEE
   121  DEAPFQEKDS GSDEDFLMED DDDSDYGSSK KKNKKMVKKS KPERKEKKMP KPRLKATVTP
   181  SPVKGKGKVG RPTASKASKE KTPSPKEEDE EPESPPEKKT STSPPPEKSG DEGSEDEAPS
   241  GED

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NUCKS1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.71
Highest tissue expression
107 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 107 nTPM
  • cerebral cortex: 102 nTPM
  • liver: 97 nTPM
  • ovary: 97 nTPM
  • tongue: 95 nTPM
  • spinal cord: 94 nTPM

Single-cell type

  • megakaryocyte progenitors: 784 nCPM
  • hepatocytes: 768 nCPM
  • monocyte progenitors: 650 nCPM
  • erythrocyte progenitors: 552 nCPM
  • megakaryocytes: 538 nCPM
  • smooth muscle cells: 514 nCPM

Immune cell

  • NK-cell: 11 nTPM
  • naive CD8 T-cell: 11 nTPM
  • naive CD4 T-cell: 10 nTPM
  • gdT-cell: 9.1 nTPM
  • MAIT T-cell: 8.8 nTPM
  • memory CD4 T-cell: 8.3 nTPM

Brain region

  • white matter: 203 nTPM
  • basal ganglia: 169 nTPM
  • spinal cord: 168 nTPM
  • medulla oblongata: 166 nTPM
  • hypothalamus: 155 nTPM
  • thalamus: 155 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.31
gnomAD pLI
0.97
gnomAD missense Z
2.09
DepMap mean gene effect
-0.04
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 13% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NUCKS1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NUCKS1 as an antibody target. Whether an autoantibody or antibody against NUCKS1 could matter depends on whether native NUCKS1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NUCKS1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NUCKS1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NUCKS1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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