Seroatlas · Human Serome Atlas

EXO1

Exonuclease 1

Also known as: EXO1_HUMAN, HEX1, hExoI

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9UQ84
Gene: EXO1
Ensembl: ENSG00000174371
Chromosome: 1
Canonical length: 846 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear bodies

OverviewNCBI Gene

This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

846 residues, UniProt reviewed canonical sequence.

>Q9UQ84|EXO1
     1  MGIQGLLQFI KEASEPIHVR KYKGQVVAVD TYCWLHKGAI ACAEKLAKGE PTDRYVGFCM
    61  KFVNMLLSHG IKPILVFDGC TLPSKKEVER SRRERRQANL LKGKQLLREG KVSEARECFT
   121  RSINITHAMA HKVIKAARSQ GVDCLVAPYE ADAQLAYLNK AGIVQAIITE DSDLLAFGCK
   181  KVILKMDQFG NGLEIDQARL GMCRQLGDVF TEEKFRYMCI LSGCDYLSSL RGIGLAKACK
   241  VLRLANNPDI VKVIKKIGHY LKMNITVPED YINGFIRANN TFLYQLVFDP IKRKLIPLNA
   301  YEDDVDPETL SYAGQYVDDS IALQIALGNK DINTFEQIDD YNPDTAMPAH SRSHSWDDKT
   361  CQKSANVSSI WHRNYSPRPE SGTVSDAPQL KENPSTVGVE RVISTKGLNL PRKSSIVKRP
   421  RSAELSEDDL LSQYSLSFTK KTKKNSSEGN KSLSFSEVFV PDLVNGPTNK KSVSTPPRTR
   481  NKFATFLQRK NEESGAVVVP GTRSRFFCSS DSTDCVSNKV SIQPLDETAV TDKENNLHES
   541  EYGDQEGKRL VDTDVARNSS DDIPNNHIPG DHIPDKATVF TDEESYSFES SKFTRTISPP
   601  TLGTLRSCFS WSGGLGDFSR TPSPSPSTAL QQFRRKSDSP TSLPENNMSD VSQLKSEESS
   661  DDESHPLREE ACSSQSQESG EFSLQSSNAS KLSQCSSKDS DSEESDCNIK LLDSQSDQTS
   721  KLRLSHFSKK DTPLRNKVPG LYKSSSADSL STTKIKPLGP ARASGLSKKP ASIQKRKHHN
   781  AENKPGLQIK LNELWKNFGF KKDSEKLPPC KKPLSPVRDN IQLTPEAEED IFNKPECGRV
   841  QRAIFQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EXO1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.51
Highest tissue expression: 16 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 16 nTPM
thymus: 10 nTPM
tonsil: 6 nTPM
lymph node: 5.4 nTPM
testis: 5.3 nTPM
rectum: 3.4 nTPM

Single-cell type

erythrocyte progenitors: 64 nCPM
monocyte progenitors: 41 nCPM
extravillous trophoblasts: 35 nCPM
megakaryocyte progenitors: 34 nCPM
migrating cytotrophoblasts: 27 nCPM
differentiating spermatogonia: 25 nCPM

Immune cell

T-reg: 0.9 nTPM
basophil: 0.8 nTPM
memory B-cell: 0.3 nTPM
naive B-cell: 0.3 nTPM
total PBMC: 0.3 nTPM
classical monocyte: 0.2 nTPM

Brain region

cerebral cortex: 1.3 nTPM
white matter: 1.2 nTPM
thalamus: 0.9 nTPM
midbrain: 0.8 nTPM
amygdala: 0.7 nTPM
basal ganglia: 0.7 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.93
gnomAD pLI: 0
gnomAD missense Z: -1.03
DepMap mean gene effect: -0.12
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

5'-3' DNA exonuclease activity
5'-3' exonuclease activity
5'-flap endonuclease activity
chromatin binding
DNA binding
exonuclease activity
flap endonuclease activity
metal ion binding
RNA-DNA hybrid ribonuclease activity
single-stranded DNA 5'-3' DNA exonuclease activity
double-stranded DNA 5'-3' DNA exonuclease activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

XPG/Rad2 endonuclease
XPG, N-terminal
XPG-I domain
Helix-hairpin-helix motif, class 2
XPG conserved site
PIN-like domain superfamily
5'-3' exonuclease, C-terminal domain superfamily
XPG N-terminal domain
XPG I-region
Exonuclease-1, H3TH domain
Exonuclease 1-like, PIN-like domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EXO1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EXO1 as an antibody target. Whether an autoantibody or antibody against EXO1 could matter depends on whether native EXO1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EXO1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EXO1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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