Seroatlas · Human Serome Atlas

EML4

Echinoderm microtubule-associated protein-like 4

Also known as: C2orf2, ELP120, EMAL4_HUMAN, ROPP120

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9HC35
Gene
EML4
Ensembl
ENSG00000143924
Chromosome
2
Canonical length
981 aa
Protein class
Cancer-related genes, Disease related genes, Predicted intracellular proteins
Subcellular location
Microtubules,Cytokinetic bridge,Primary cilium,Cytosol,Perinuclear theca,Calyx,Principal piece,Annulus
Quaternary structure
Homotrimer

OverviewNCBI Gene

This gene is a member of the echinoderm microtubule associated protein-like family. The encoded WD-repeat protein may be involved in microtubule formation. Abnormal fusion of parts of this gene with portions of the anaplastic lymphoma receptor tyrosine kinase gene, which generates EML4-ALK fusion transcripts, is one of the primary mutations associated with non-small cell lung cancer. Alternative splicing of this gene results in two transcript variants. [provided by RefSeq, Jan 2015]

Canonical amino-acid sequenceUniProt

981 residues, UniProt reviewed canonical sequence.

>Q9HC35|EML4
     1  MDGFAGSLDD SISAASTSDV QDRLSALESR VQQQEDEITV LKAALADVLR RLAISEDHVA
    61  SVKKSVSSKG QPSPRAVIPM SCITNGSGAN RKPSHTSAVS IAGKETLSSA AKSGTEKKKE
   121  KPQGQREKKE ESHSNDQSPQ IRASPSPQPS SQPLQIHRQT PESKNATPTK SIKRPSPAEK
   181  SHNSWENSDD SRNKLSKIPS TPKLIPKVTK TADKHKDVII NQEGEYIKMF MRGRPITMFI
   241  PSDVDNYDDI RTELPPEKLK LEWAYGYRGK DCRANVYLLP TGKIVYFIAS VVVLFNYEER
   301  TQRHYLGHTD CVKCLAIHPD KIRIATGQIA GVDKDGRPLQ PHVRVWDSVT LSTLQIIGLG
   361  TFERGVGCLD FSKADSGVHL CIIDDSNEHM LTVWDWQKKA KGAEIKTTNE VVLAVEFHPT
   421  DANTIITCGK SHIFFWTWSG NSLTRKQGIF GKYEKPKFVQ CLAFLGNGDV LTGDSGGVML
   481  IWSKTTVEPT PGKGPKGVYQ ISKQIKAHDG SVFTLCQMRN GMLLTGGGKD RKIILWDHDL
   541  NPEREIEVPD QYGTIRAVAE GKADQFLVGT SRNFILRGTF NDGFQIEVQG HTDELWGLAT
   601  HPFKDLLLTC AQDRQVCLWN SMEHRLEWTR LVDEPGHCAD FHPSGTVVAI GTHSGRWFVL
   661  DAETRDLVSI HTDGNEQLSV MRYSIDGTFL AVGSHDNFIY LYVVSENGRK YSRYGRCTGH
   721  SSYITHLDWS PDNKYIMSNS GDYEILYWDI PNGCKLIRNR SDCKDIDWTT YTCVLGFQVF
   781  GVWPEGSDGT DINALVRSHN RKVIAVADDF CKVHLFQYPC SKAKAPSHKY SAHSSHVTNV
   841  SFTHNDSHLI STGGKDMSII QWKLVEKLSL PQNETVADTT LTKAPVSSTE SVIQSNTPTP
   901  PPSQPLNETA EEESRISSSP TLLENSLEQT VEPSEDHSEE ESEEGSGDLG EPLYEEPCNE
   961  ISKEQAKATL LEDQQDPSPS S

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EML4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
39 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 39 nTPM
  • duodenum: 37 nTPM
  • liver: 36 nTPM
  • small intestine: 34 nTPM
  • placenta: 34 nTPM
  • appendix: 31 nTPM

Single-cell type

  • innate lymphoid cells: 1,053 nCPM
  • t-cells: 899 nCPM
  • neutrophils: 800 nCPM
  • neutrophil progenitors: 732 nCPM
  • urothelial cells: 685 nCPM
  • pituicytes/fscs: 673 nCPM

Immune cell

  • T-reg: 22 nTPM
  • MAIT T-cell: 19 nTPM
  • memory CD4 T-cell: 15 nTPM
  • memory CD8 T-cell: 15 nTPM
  • non-classical monocyte: 15 nTPM
  • memory B-cell: 14 nTPM

Brain region

  • choroid plexus: 17 nTPM
  • thalamus: 17 nTPM
  • white matter: 15 nTPM
  • basal ganglia: 14 nTPM
  • medulla oblongata: 13 nTPM
  • spinal cord: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.46
gnomAD pLI
0
gnomAD missense Z
-0.37
DepMap mean gene effect
-0.11
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EML4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EML4 as an antibody target. Whether an autoantibody or antibody against EML4 could matter depends on whether native EML4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EML4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EML4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EML4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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