Seroatlas · Human Serome Atlas

EAF1

ELL-associated factor 1

Also known as: EAF1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96JC9
Gene: EAF1
Ensembl: ENSG00000144597
Chromosome: 3
Canonical length: 268 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear bodies,Vesicles

OverviewNCBI Gene

Enables transcription elongation factor activity. Involved in regulation of transcription elongation by RNA polymerase II. Located in intercellular bridge and nuclear body. Part of transcription elongation factor complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

268 residues, UniProt reviewed canonical sequence.

>Q96JC9|EAF1
     1  MNGTANPLLD REEHCLRLGE SFEKRPRASF HTIRYDFKPA SIDTSCEGEL QVGKGDEVTI
    61  TLPHIPGSTP PMTVFKGNKR PYQKDCVLII NHDTGEYVLE KLSSSIQVKK TRAEGSSKIQ
   121  ARMEQQPTRP PQTSQPPPPP PPMPFRAPTK PPVGPKTSPL KDNPSPEPQL DDIKRELRAE
   181  VDIIEQMSSS SGSSSSDSES SSGSDDDSSS SGGEDNGPAS PPQPSHQQPY NSRPAVANGT
   241  SRPQGSNQLM NTLRNDLQLS ESGSDSDD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EAF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.56
Highest tissue expression: 54 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 54 nTPM
liver: 18 nTPM
cerebral cortex: 13 nTPM
adipose tissue: 12 nTPM
parathyroid gland: 11 nTPM
skin: 11 nTPM

Single-cell type

late spermatids: 617 nCPM
early spermatids: 350 nCPM
monocytes: 124 nCPM
kupffer cells: 64 nCPM
monocyte progenitors: 59 nCPM
neutrophils: 59 nCPM

Immune cell

MAIT T-cell: 14 nTPM
gdT-cell: 11 nTPM
T-reg: 11 nTPM
plasmacytoid DC: 9.8 nTPM
memory CD4 T-cell: 9.2 nTPM
naive CD8 T-cell: 8.7 nTPM

Brain region

pons: 29 nTPM
hypothalamus: 26 nTPM
thalamus: 25 nTPM
midbrain: 24 nTPM
hippocampal formation: 24 nTPM
medulla oblongata: 23 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.77
gnomAD pLI: 0.06
gnomAD missense Z: 1.01
DepMap mean gene effect: -0.43
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

transcription elongation factor activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of EAF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EAF1 as an antibody target. Whether an autoantibody or antibody against EAF1 could matter depends on whether native EAF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EAF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EAF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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