DDX39A

Protein identityUniProt · HPA

UniProt accession

O00148

Gene

DDX39A

Ensembl

ENSG00000123136

Chromosome

19

Canonical length

427 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Nuclear speckles

OverviewNCBI Gene

This gene encodes a member of the DEAD box protein family. These proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is thought to play a role in the prognosis of patients with gastrointestinal stromal tumors. A pseudogene of this gene is present on chromosome 13. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]

Canonical amino-acid sequenceUniProt

427 residues, UniProt reviewed canonical sequence.

>O00148|DDX39A
     1  MAEQDVENDL LDYDEEEEPQ APQESTPAPP KKDIKGSYVS IHSSGFRDFL LKPELLRAIV
    61  DCGFEHPSEV QHECIPQAIL GMDVLCQAKS GMGKTAVFVL ATLQQIEPVN GQVTVLVMCH
   121  TRELAFQISK EYERFSKYMP SVKVSVFFGG LSIKKDEEVL KKNCPHVVVG TPGRILALVR
   181  NRSFSLKNVK HFVLDECDKM LEQLDMRRDV QEIFRLTPHE KQCMMFSATL SKDIRPVCRK
   241  FMQDPMEVFV DDETKLTLHG LQQYYVKLKD SEKNRKLFDL LDVLEFNQVI IFVKSVQRCM
   301  ALAQLLVEQN FPAIAIHRGM AQEERLSRYQ QFKDFQRRIL VATNLFGRGM DIERVNIVFN
   361  YDMPEDSDTY LHRVARAGRF GTKGLAITFV SDENDAKILN DVQDRFEVNV AELPEEIDIS
   421  TYIEQSR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DDX39A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.27

Highest tissue expression

156 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.45

gnomAD pLI

0.6

gnomAD missense Z

2.94

DepMap mean gene effect

-0.18

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• mRNA export from nucleus
• mRNA splicing, via spliceosome
• negative regulation of innate immune response
• negative regulation of RNA export from nucleus
• RNA export from nucleus

Molecular functions

• ATP binding
• ATP hydrolysis activity
• identical protein binding
• mRNA binding
• RNA binding
• RNA helicase activity

Cellular components

• cytoplasm
• membrane
• nuclear speck
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Helicase, C-terminal domain-like
• DEAD/DEAH-box helicase domain
• Helicase superfamily 1/2, ATP-binding domain
• RNA helicase, DEAD-box type, Q motif
• P-loop containing nucleoside triphosphate hydrolase
• DEAD/DEAH box helicase
• Helicase conserved C-terminal domain

KeywordsUniProt

• Acetylation
• ATP-binding
• Cytoplasm
• Helicase
• Hydrolase
• Isopeptide bond
• mRNA processing
• mRNA splicing
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• RNA-binding
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of DDX39A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ECD
• MX1
• PHAX
• SARNP
• DDX39B