Seroatlas · Human Serome Atlas

NACA

Nascent polypeptide-associated complex subunit alpha, muscle-specific form

Also known as: NACA1, NACAM_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
E9PAV3
Gene
NACA
Ensembl
ENSG00000196531
Chromosome
12
Canonical length
2078 aa
Protein class
Plasma proteins, Predicted intracellular proteins
Subcellular location
Cytosol

OverviewNCBI Gene

This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes. [provided by RefSeq, Feb 2016]

Canonical amino-acid sequenceUniProt

2078 residues, UniProt reviewed canonical sequence.

>E9PAV3|NACA
     1  MPGEATETVP ATEQELPQPQ AETAVLPMSS ALSVTAALGQ PGPTLPPPCS PAPQQCPLSA
    61  ANQASPFPSP STIASTPLEV PFPQSSSGTA LPLGTAPEAP TFLPNLIGPP ISPAALALAS
   121  PMIAPTLKGT PSSSAPLALV ALAPHSVQKS SAFPPNLLTS PPSVAVAESG SVITLSAPIA
   181  PSEPKTNLNK VPSEVVPNPK GTPSPPCIVS TVPYHCVTPM ASIQSGVASL PQTTPTTTLA
   241  IASPQVKDTT ISSVLISPQN PGSLSLKGPV SPPAALSLST QSLPVVTSSQ KTAGPNTPPD
   301  FPISLGSHLA PLHQSSFGSV QLLGQTGPSA LSDPTVKTIS VDHSSTGASY PSQRSVIPPL
   361  PSRNEVVPAT VAAFPVVAPS VDKGPSTISS ITCSPSGSLN VATSFSLSPT TSLILKSSPN
   421  ATYHYPLVAQ MPVSSVGTTP LVVTNPCTIA AAPTTTFEVA TCVSPPMSSG PISNIEPTSP
   481  AALVMAPVAP KEPSTQVATT LRIPVSPPLP DPEDLKNLPS SVLVKFPTQK DLQTVPASLE
   541  GAPFSPAQAG LTTKKDPTVL PLVQAAPKNS PSFQSTSSSP EIPLSPEATL AKKSLGEPLP
   601  IGKPASSMTS PLGVNSSASV IKTDSYAGPD SAGPLLKSSL ITPTVAAFPL ESADPAGVAP
   661  TTAKGTSTYT TTASPFLEGT VSLAPKNHPV KEGTLTTLPL VPTASENCPV APSPQNTCAP
   721  LATLVLAPEI PKSVPSPSLP PAGTPPGTKK VDGISHTSAL APVASSPKEC PTEDSGASAT
   781  ASSKGTLTYL ADSPSPLGVS VSPQTKRPPT KKGSAGPDTP IGNLSSPVSP VEASFLPENS
   841  LSFQGSKDSP ATTHSPTPPS PKGAPTPSAV TPLSPKGVTL PPKETPTPSV VNLPFPKEGP
   901  ATPAPKQAPA LSMTSSSPKK ARATPAPKGI PASPSPKGAP TPPAATPPSP KGGPATPSPK
   961  WAPTPPAATP PSPKGGPATP SPKGAPTPPA ATPPSPKGGP ATPSPKGAPT PPAVTPPSPK
  1021  GSPAATPFPK GASTPPAATP PSPKGSPAAT PLPKGAPTTP AATLPSPKGG PATPSLKGAP
  1081  TPPAATPPSP KGGPATPSPK GAPMPPAATP PSPKGGLATP PHKGAPTTPA ATPPSPKGGL
  1141  ATPPPKGAPT TPAATPPSPK GGLATPPPKG APTTPAATPP SPKGGLATPS PKGAPTTPAA
  1201  TPPSPKGGLA TPSPKGAPTT PAATPPSPKG GLATPSPKGA PTTPAATPPS PKGGPATPPP
  1261  KGAPTPPAAT PPSLKGGLAT PPHKGAPNPA VVTPPSPKGG PATSPPKGAP TPPAATPPSP
  1321  KGSPGTPPPK GAPTPPAVTP PSPKGTPTLP ATTPSSKGGP TTPSSKEGPT PPAATPSHKG
  1381  GPAMTPPSPK RGPAIPSPKG DPTSPAVIPL SPKKAPATPV TREGAATPSK GDLTPPAVTP
  1441  VSLKKAPATS APKGGPATPS SKGDPTLPAV TPPSPKEPPA PKQVATSSSP KKAPATPAPM
  1501  GAPTLPAVIP SSPKEVPATP SSRRDPIAPT ATLLSKKTPA TLAPKEALIP PAMTVPSPKK
  1561  TPAIPTPKEA PATPSSKEAS SPPAVTPSTY KGAPSPKELL IPPAVTSPSP KEAPTPPAVT
  1621  PPSPEKGPAT PAPKGTPTSP PVTPSSLKDS PTSPASVTCK MGATVPQASK GLPAKKGPTA
  1681  LKEVLVAPAP ESTPIITAPT RKGPQTKKSS ATSPPICPDP SAKNGSKGPL STVAPAPLLP
  1741  VQKDSSKTAK GKDASHSPKG PLAPPESKAS TPLTAAAFEK VLPKPESASV SAAPSPPVSL
  1801  PLAPSPVPTL PPKQQFLPSS PGLVLESPSK PLAPADEDEL LPLIPPEPIS GGVPFQSVLV
  1861  NMPTPKSAGI PVPTPSAKQP VTKNNKGSGT ESDSDESVPE LEEQDSTQAT TQQAQLAAAA
  1921  EIDEEPVSKA KQSRSEKKAR KAMSKLGLRQ VTGVTRVTIR KSKNILFVIT KPDVYKSPAS
  1981  DTYIVFGEAK IEDLSQQAQL AAAEKFKVQG EAVSNIQENT QTPTVQEESE EEEVDETGVE
  2041  VKDIELVMSQ ANVSRAKAVR ALKNNSNDIV NAIMELTM

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NACA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.68
Highest tissue expression
2,306 nTPM

Expression across tissuesHPA

Tissue

  • ovary: 2,306 nTPM
  • skin: 1,619 nTPM
  • cervix: 1,485 nTPM
  • esophagus: 1,459 nTPM
  • bone marrow: 1,437 nTPM
  • vagina: 1,412 nTPM

Single-cell type

  • esophageal apical cells: 7,147 nCPM
  • esophageal suprabasal cells: 5,431 nCPM
  • esophageal basal cells: 4,476 nCPM
  • extravillous trophoblasts: 3,610 nCPM
  • decidual stromal cells: 3,565 nCPM
  • breast secretory cells: 2,908 nCPM

Immune cell

  • total PBMC: 1,953 nTPM
  • non-classical monocyte: 1,852 nTPM
  • intermediate monocyte: 1,373 nTPM
  • plasmacytoid DC: 1,086 nTPM
  • myeloid DC: 1,009 nTPM
  • classical monocyte: 989 nTPM

Brain region

  • spinal cord: 344 nTPM
  • white matter: 323 nTPM
  • hypothalamus: 302 nTPM
  • medulla oblongata: 287 nTPM
  • cerebellum: 259 nTPM
  • pons: 256 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.7
gnomAD pLI
0
gnomAD missense Z
0.9
DepMap mean gene effect
-1.28
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NACA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NACA as an antibody target. Whether an autoantibody or antibody against NACA could matter depends on whether native NACA is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NACA is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Source-annotated serology context

The source annotations explicitly mention antibody, autoantibody, autoantigen, or autoimmune context. This is biological context, not study-specific reactivity.

  • This protein is an IgE autoantigen in atopic dermatitis patients.

Canonical record: https://seroatlas.com/gene/NACA. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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