Seroatlas · Human Serome Atlas

ARFIP1

Arfaptin-1

Also known as: ARFP1_HUMAN, HSU52521

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P53367
Gene
ARFIP1
Ensembl
ENSG00000164144
Chromosome
4
Canonical length
373 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Golgi apparatus,Vesicles
Quaternary structure
Homodimer

OverviewNCBI Gene

Enables phosphatidylinositol-4-phosphate binding activity. Involved in negative regulation of retrograde transport, endosome to Golgi. Acts upstream of or within intracellular protein transport and regulation of protein secretion. Located in Golgi membrane; cytosol; and trans-Golgi network membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

373 residues, UniProt reviewed canonical sequence.

>P53367|ARFIP1
     1  MAQESPKNSA AEIPVTSNGE VDDSREHSFN RDLKHSLPSG LGLSETQITS HGFDNTKEGV
    61  IEAGAFQGSP APPLPSVMSP SRVAASRLAQ QGSDLIVPAG GQRTQTKSGP VILADEIKNP
   121  AMEKLELVRK WSLNTYKCTR QIISEKLGRG SRTVDLELEA QIDILRDNKK KYENILKLAQ
   181  TLSTQLFQMV HTQRQLGDAF ADLSLKSLEL HEEFGYNADT QKLLAKNGET LLGAINFFIA
   241  SVNTLVNKTI EDTLMTVKQY ESARIEYDAY RTDLEELNLG PRDANTLPKI EQSQHLFQAH
   301  KEKYDKMRND VSVKLKFLEE NKVKVLHNQL VLFHNAIAAY FAGNQKQLEQ TLKQFHIKLK
   361  TPGVDAPSWL EEQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARFIP1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.47
Highest tissue expression
26 nTPM

Expression across tissuesHPA

Tissue

  • colon: 26 nTPM
  • rectum: 26 nTPM
  • breast: 22 nTPM
  • urinary bladder: 22 nTPM
  • duodenum: 22 nTPM
  • stomach: 22 nTPM

Single-cell type

  • neutrophils: 247 nCPM
  • pituitary stem cells: 230 nCPM
  • microglia: 198 nCPM
  • choroid plexus epithelial cells: 167 nCPM
  • cone photoreceptor cells: 155 nCPM
  • rod photoreceptor cells: 152 nCPM

Immune cell

  • neutrophil: 82 nTPM
  • eosinophil: 42 nTPM
  • basophil: 32 nTPM
  • intermediate monocyte: 31 nTPM
  • non-classical monocyte: 31 nTPM
  • NK-cell: 29 nTPM

Brain region

  • white matter: 21 nTPM
  • choroid plexus: 19 nTPM
  • cerebellum: 19 nTPM
  • medulla oblongata: 18 nTPM
  • spinal cord: 18 nTPM
  • hypothalamus: 18 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.94
gnomAD pLI
0
gnomAD missense Z
1.08
DepMap mean gene effect
0.04
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARFIP1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARFIP1 as an antibody target. Whether an autoantibody or antibody against ARFIP1 could matter depends on whether native ARFIP1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARFIP1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ARFIP1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ARFIP1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...