Seroatlas · Human Serome Atlas

ARF5

ADP-ribosylation factor 5

Also known as: ARF5_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P84085
Gene: ARF5
Ensembl: ENSG00000004059
Chromosome: 7
Canonical length: 180 aa
Protein class: Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. [provided by RefSeq, Dec 2010]

Canonical amino-acid sequenceUniProt

180 residues, UniProt reviewed canonical sequence.

>P84085|ARF5
     1  MGLTVSALFS RIFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN
    61  ICFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV QESADELQKM LQEDELRDAV
   121  LLVFANKQDM PNAMPVSELT DKLGLQHLRS RTWYVQATCA TQGTGLYDGL DWLSHELSKR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARF5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.28
Highest tissue expression: 249 nTPM

Expression across tissuesHPA

Tissue

esophagus: 249 nTPM
cerebral cortex: 222 nTPM
skin: 215 nTPM
basal ganglia: 195 nTPM
pancreas: 182 nTPM
amygdala: 177 nTPM

Single-cell type

esophageal apical cells: 280 nCPM
oocytes: 263 nCPM
epididymal principal cells: 230 nCPM
esophageal suprabasal cells: 207 nCPM
breast myoepithelial cells: 195 nCPM
decidual stromal cells: 178 nCPM

Immune cell

neutrophil: 450 nTPM
myeloid DC: 410 nTPM
total PBMC: 382 nTPM
classical monocyte: 322 nTPM
basophil: 290 nTPM
eosinophil: 280 nTPM

Brain region

cerebral cortex: 134 nTPM
basal ganglia: 123 nTPM
hippocampal formation: 112 nTPM
amygdala: 106 nTPM
hypothalamus: 104 nTPM
thalamus: 100 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.33
gnomAD pLI: 0.94
gnomAD missense Z: 2.28
DepMap mean gene effect: 0.03
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARF5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARF5 as an antibody target. Whether an autoantibody or antibody against ARF5 could matter depends on whether native ARF5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARF5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ARF5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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