Seroatlas · Human Serome Atlas

ARF4

ADP-ribosylation factor 4

Also known as: ARF2, ARF4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P18085
Gene
ARF4
Ensembl
ENSG00000168374
Chromosome
3
Canonical length
180 aa
Protein class
Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

180 residues, UniProt reviewed canonical sequence.

>P18085|ARF4
     1  MGLTISSLFS RLFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN
    61  ICFTVWDVGG QDRIRPLWKH YFQNTQGLIF VVDSNDRERI QEVADELQKM LLVDELRDAV
   121  LLLFANKQDL PNAMAISEMT DKLGLQSLRN RTWYVQATCA TQGTGLYEGL DWLSNELSKR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARF4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.28
Highest tissue expression
214 nTPM

Expression across tissuesHPA

Tissue

  • epididymis: 214 nTPM
  • cervix: 196 nTPM
  • urinary bladder: 190 nTPM
  • rectum: 185 nTPM
  • salivary gland: 171 nTPM
  • placenta: 171 nTPM

Single-cell type

  • esophageal apical cells: 1,192 nCPM
  • syncytiotrophoblasts: 947 nCPM
  • epididymal principal cells: 688 nCPM
  • extravillous trophoblasts: 681 nCPM
  • neutrophils: 655 nCPM
  • enterocytes: 611 nCPM

Immune cell

  • basophil: 250 nTPM
  • plasmacytoid DC: 109 nTPM
  • neutrophil: 103 nTPM
  • eosinophil: 96 nTPM
  • total PBMC: 91 nTPM
  • classical monocyte: 85 nTPM

Brain region

  • choroid plexus: 95 nTPM
  • hypothalamus: 72 nTPM
  • midbrain: 68 nTPM
  • white matter: 67 nTPM
  • spinal cord: 66 nTPM
  • pons: 62 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.3
gnomAD pLI
0.96
gnomAD missense Z
2.11
DepMap mean gene effect
-0.69
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARF4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARF4 as an antibody target. Whether an autoantibody or antibody against ARF4 could matter depends on whether native ARF4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARF4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ARF4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ARF4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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