Seroatlas · Human Serome Atlas

ALKBH3

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3

Also known as: ALKB3_HUMAN, DEPC-1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96Q83
Gene
ALKBH3
Ensembl
ENSG00000166199
Chromosome
11
Canonical length
286 aa
Protein class
Enzymes, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Mitochondria

OverviewNCBI Gene

The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

286 residues, UniProt reviewed canonical sequence.

>Q96Q83|ALKBH3
     1  MEEKRRRARV QGAWAAPVKS QAIAQPATTA KSHLHQKPGQ TWKNKEHHLS DREFVFKEPQ
    61  QVVRRAPEPR VIDREGVYEI SLSPTGVSRV CLYPGFVDVK EADWILEQLC QDVPWKQRTG
   121  IREDITYQQP RLTAWYGELP YTYSRITMEP NPHWHPVLRT LKNRIEENTG HTFNSLLCNL
   181  YRNEKDSVDW HSDDEPSLGR CPIIASLSFG ATRTFEMRKK PPPEENGDYT YVERVKIPLD
   241  HGTLLIMEGA TQADWQHRVP KEYHSREPRV NLTFRTVYPD PRGAPW

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ALKBH3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
30 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 30 nTPM
  • tongue: 24 nTPM
  • epididymis: 23 nTPM
  • choroid plexus: 22 nTPM
  • pancreas: 21 nTPM
  • ovary: 21 nTPM

Single-cell type

  • early spermatids: 131 nCPM
  • late spermatids: 131 nCPM
  • late primary spermatocytes: 64 nCPM
  • cardiomyocytes: 55 nCPM
  • esophageal suprabasal cells: 40 nCPM
  • ocular epithelial cells: 38 nCPM

Immune cell

  • basophil: 35 nTPM
  • non-classical monocyte: 30 nTPM
  • naive CD4 T-cell: 25 nTPM
  • NK-cell: 25 nTPM
  • myeloid DC: 24 nTPM
  • naive CD8 T-cell: 23 nTPM

Brain region

  • cerebellum: 20 nTPM
  • white matter: 18 nTPM
  • cerebral cortex: 17 nTPM
  • basal ganglia: 15 nTPM
  • choroid plexus: 15 nTPM
  • medulla oblongata: 15 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.32
gnomAD pLI
0
gnomAD missense Z
0.08
DepMap mean gene effect
-0.07
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ALKBH3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ALKBH3 as an antibody target. Whether an autoantibody or antibody against ALKBH3 could matter depends on whether native ALKBH3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ALKBH3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ALKBH3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ALKBH3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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