TFEB
Transcription factor EB
Also known as: bHLHe35, TCFEB, TFEB_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P19484
- Gene
- TFEB
- Ensembl
- ENSG00000112561
- Chromosome
- 6
- Canonical length
- 476 aa
- Protein class
- Cancer-related genes, Predicted intracellular proteins, Transcription factors
- Subcellular location
- Cytosol
- Quaternary structure
- Homodimer
OverviewNCBI Gene
Enables DNA-binding transcription factor activity; enzyme binding activity; and transcription cis-regulatory region binding activity. Involved in several processes, including antibacterial innate immune response; cellular response to amino acid starvation; and positive regulation of metabolic process. Located in cytosol and lysosomal membrane. Is active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
476 residues, UniProt reviewed canonical sequence.
>P19484|TFEB
1 MASRIGLRMQ LMREQAQQEE QRERMQQQAV MHYMQQQQQQ QQQQLGGPPT PAINTPVHFQ
61 SPPPVPGEVL KVQSYLENPT SYHLQQSQHQ KVREYLSETY GNKFAAHISP AQGSPKPPPA
121 ASPGVRAGHV LSSSAGNSAP NSPMAMLHIG SNPERELDDV IDNIMRLDDV LGYINPEMQM
181 PNTLPLSSSH LNVYSSDPQV TASLVGVTSS SCPADLTQKR ELTDAESRAL AKERQKKDNH
241 NLIERRRRFN INDRIKELGM LIPKANDLDV RWNKGTILKA SVDYIRRMQK DLQKSRELEN
301 HSRRLEMTNK QLWLRIQELE MQARVHGLPT TSPSGMNMAE LAQQVVKQEL PSEEGPGEAL
361 MLGAEVPDPE PLPALPPQAP LPLPTQPPSP FHHLDFSHSL SFGGREDEGP PGYPEPLAPG
421 HGSPFPSLSK KDLDLMLLDD SLLPLASDPL LSTMSPEASK ASSRRSSFSM EEGDVLLocalizationUniProt · AlphaFold · HPA
Whether an antibody against TFEB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Other membrane
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.62
- Highest tissue expression
- 77 nTPM
Expression across tissuesHPA
Tissue
- skeletal muscle: 77 nTPM
- tongue: 39 nTPM
- spinal cord: 38 nTPM
- spleen: 36 nTPM
- heart muscle: 27 nTPM
- lymph node: 25 nTPM
Single-cell type
- oligodendrocytes: 172 nCPM
- syncytiotrophoblasts: 139 nCPM
- neutrophils: 138 nCPM
- renal collecting duct intercalated cells: 112 nCPM
- b-cells: 111 nCPM
- renal connecting tubule cells: 82 nCPM
Immune cell
- neutrophil: 48 nTPM
- memory B-cell: 34 nTPM
- naive B-cell: 23 nTPM
- eosinophil: 17 nTPM
- intermediate monocyte: 16 nTPM
- classical monocyte: 16 nTPM
Brain region
- white matter: 73 nTPM
- medulla oblongata: 58 nTPM
- basal ganglia: 54 nTPM
- midbrain: 50 nTPM
- cerebral cortex: 48 nTPM
- thalamus: 45 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.38
- gnomAD pLI
- 0.9
- gnomAD missense Z
- 1.77
- DepMap mean gene effect
- -0.02
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- adaptive immune response
- antibacterial innate immune response
- autophagy
- cellular response to amino acid starvation
- cellular response to starvation
- defense response to Gram-negative bacterium
- embryonic placenta development
- humoral immune response
- lysosome localization
- lysosome organization
- positive regulation of autophagy
- positive regulation of DNA-templated transcription
- positive regulation of transcription by RNA polymerase II
- regulation of DNA-templated transcription
- regulation of lysosome organization
- regulation of transcription by RNA polymerase II
Molecular functions
- DNA-binding transcription activator activity, RNA polymerase II-specific
- DNA-binding transcription factor activity
- DNA-binding transcription factor activity, RNA polymerase II-specific
- enzyme binding
- protein heterodimerization activity
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- sequence-specific double-stranded DNA binding
- transcription cis-regulatory region binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Myc-type, basic helix-loop-helix (bHLH) domain
- MiT/TFE transcription factors, C-terminal
- MiT/TFE transcription factors, N-terminal
- Helix-loop-helix DNA-binding domain superfamily
- Helix-loop-helix DNA-binding domain
- Domain of unknown function (DUF3371)
- MITF/TFEB/TFEC/TFE3 N-terminus
- Transcription factor EB, bHLHzip domain
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of TFEB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads TFEB as an antibody target. Whether an autoantibody or antibody against TFEB could matter depends on whether native TFEB is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
TFEB is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label TFEB as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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