Seroatlas · Human Serome Atlas

USP49

Ubiquitin carboxyl-terminal hydrolase 49

Also known as: MGC20741, UBP49_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q70CQ1
Gene
USP49
Ensembl
ENSG00000164663
Chromosome
6
Canonical length
688 aa
Protein class
Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

Enables histone binding activity and peptidase activity. Involved in mRNA splicing, via spliceosome; negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; and protein deubiquitination. Predicted to be located in nucleoplasm. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

688 residues, UniProt reviewed canonical sequence.

>Q70CQ1|USP49
     1  MDRCKHVGRL RLAQDHSILN PQKWCCLECA TTESVWACLK CSHVACGRYI EDHALKHFEE
    61  TGHPLAMEVR DLYVFCYLCK DYVLNDNPEG DLKLLRSSLL AVRGQKQDTP VRRGRTLRSM
   121  ASGEDVVLPQ RAPQGQPQML TALWYRRQRL LARTLRLWFE KSSRGQAKLE QRRQEEALER
   181  KKEEARRRRR EVKRRLLEEL ASTPPRKSAR LLLHTPRDAG PAASRPAALP TSRRVPAATL
   241  KLRRQPAMAP GVTGLRNLGN TCYMNSILQV LSHLQKFREC FLNLDPSKTE HLFPKATNGK
   301  TQLSGKPTNS SATELSLRND RAEACEREGF CWNGRASISR SLELIQNKEP SSKHISLCRE
   361  LHTLFRVMWS GKWALVSPFA MLHSVWSLIP AFRGYDQQDA QEFLCELLHK VQQELESEGT
   421  TRRILIPFSQ RKLTKQVLKV VNTIFHGQLL SQVTCISCNY KSNTIEPFWD LSLEFPERYH
   481  CIEKGFVPLN QTECLLTEML AKFTETEALE GRIYACDQCN SKRRKSNPKP LVLSEARKQL
   541  MIYRLPQVLR LHLKRFRWSG RNHREKIGVH VVFDQVLTME PYCCRDMLSS LDKETFAYDL
   601  SAVVMHHGKG FGSGHYTAYC YNTEGGFWVH CNDSKLNVCS VEEVCKTQAY ILFYTQRTVQ
   661  GNARISETHL QAQVQSSNND EGRPQTFS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against USP49 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.4
Highest tissue expression
8.3 nTPM

Expression across tissuesHPA

Tissue

  • retina: 8.3 nTPM
  • thymus: 6 nTPM
  • bone marrow: 4.7 nTPM
  • testis: 4.1 nTPM
  • thyroid gland: 3.9 nTPM
  • cerebral cortex: 3.8 nTPM

Single-cell type

  • ependymal cells: 131 nCPM
  • oligodendrocyte progenitor cells: 71 nCPM
  • brain inhibitory neurons: 60 nCPM
  • brain excitatory neurons: 58 nCPM
  • choroid plexus epithelial cells: 55 nCPM
  • other brain neurons: 52 nCPM

Immune cell

  • basophil: 3.6 nTPM
  • neutrophil: 0.9 nTPM
  • non-classical monocyte: 0.5 nTPM
  • classical monocyte: 0.4 nTPM
  • eosinophil: 0.4 nTPM
  • memory B-cell: 0.4 nTPM

Brain region

  • cerebellum: 20 nTPM
  • amygdala: 17 nTPM
  • midbrain: 17 nTPM
  • hypothalamus: 17 nTPM
  • basal ganglia: 16 nTPM
  • cerebral cortex: 16 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.35
gnomAD pLI
0.94
gnomAD missense Z
2.62
DepMap mean gene effect
0.06
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of USP49 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads USP49 as an antibody target. Whether an autoantibody or antibody against USP49 could matter depends on whether native USP49 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

USP49 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label USP49 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/USP49. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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