Seroatlas · Human Serome Atlas

TIFA

TRAF-interacting protein with FHA domain-containing protein A

Also known as: MGC20791, T2BP, T6BP, TIFA_HUMAN, TIFAA

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96CG3
Gene
TIFA
Ensembl
ENSG00000145365
Chromosome
4
Canonical length
184 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nucleoli,Cytosol
Quaternary structure
Homooligomer

OverviewNCBI Gene

This gene encodes an adapter protein involved in adaptive and innate immunity. This protein includes a forkhead-associated (FHA) domain that specifically binds to phosphorylated serine and threonine residues. In response to bacterial infection, the encoded host cell protein undergoes an intermolecular interaction between the FHA domain and a phosphorylated threonine that leads to protein oligomerization and stimulation of the NF-kappa B and other downstream signaling pathways. This protein exhibits reduced expression in hepatocellular carcinoma and may suppress hepatocellular carcinoma progression. This protein may also play a role in the DNA damage response. [provided by RefSeq, Jun 2018]

Canonical amino-acid sequenceUniProt

184 residues, UniProt reviewed canonical sequence.

>Q96CG3|TIFA
     1  MTSFEDADTE ETVTCLQMTV YHPGQLQCGI FQSISFNREK LPSSEVVKFG RNSNICHYTF
    61  QDKQVSRVQF SLQLFKKFNS SVLSFEIKNM SKKTNLIVDS RELGYLNKMD LPYRCMVRFG
   121  EYQFLMEKED GESLEFFETQ FILSPRSLLQ ENNWPPHRPI PEYGTYSLCS SQSSSPTEMD
   181  ENES

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TIFA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.36
Highest tissue expression
39 nTPM

Expression across tissuesHPA

Tissue

  • pancreas: 39 nTPM
  • bone marrow: 38 nTPM
  • salivary gland: 33 nTPM
  • tonsil: 32 nTPM
  • lymph node: 30 nTPM
  • thyroid gland: 20 nTPM

Single-cell type

  • pancreatic acinar cells: 131 nCPM
  • esophageal apical cells: 128 nCPM
  • plasma cells: 121 nCPM
  • syncytiotrophoblasts: 121 nCPM
  • epididymal basal cells: 74 nCPM
  • salivary acinar cells: 72 nCPM

Immune cell

  • naive B-cell: 41 nTPM
  • memory B-cell: 38 nTPM
  • T-reg: 34 nTPM
  • neutrophil: 22 nTPM
  • memory CD4 T-cell: 20 nTPM
  • memory CD8 T-cell: 19 nTPM

Brain region

  • choroid plexus: 7.9 nTPM
  • cerebellum: 7.7 nTPM
  • pons: 4.2 nTPM
  • thalamus: 3.9 nTPM
  • hypothalamus: 3.8 nTPM
  • hippocampal formation: 3.7 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.15
gnomAD pLI
0.13
gnomAD missense Z
0.89
DepMap mean gene effect
0.1
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TIFA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TIFA as an antibody target. Whether an autoantibody or antibody against TIFA could matter depends on whether native TIFA is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TIFA is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TIFA as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TIFA. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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