SLIT1

Protein identityUniProt · HPA

UniProt accession

O75093

Gene

SLIT1

Ensembl

ENSG00000187122

Chromosome

10

Canonical length

1534 aa

Protein class

Predicted secreted proteins

Subcellular location

Plasma membrane

Secretome location

Secreted in brain

OverviewNCBI Gene

Enables Roundabout binding activity. Involved in axon extension involved in axon guidance; motor neuron axon guidance; and negative chemotaxis. Predicted to be located in extracellular region. Predicted to be active in extracellular space. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1534 residues, UniProt reviewed canonical sequence.

>O75093|SLIT1
     1  MALTPGWGSS AGPVRPELWL LLWAAAWRLG ASACPALCTC TGTTVDCHGT GLQAIPKNIP
    61  RNTERLELNG NNITRIHKND FAGLKQLRVL QLMENQIGAV ERGAFDDMKE LERLRLNRNQ
   121  LHMLPELLFQ NNQALSRLDL SENAIQAIPR KAFRGATDLK NLQLDKNQIS CIEEGAFRAL
   181  RGLEVLTLNN NNITTIPVSS FNHMPKLRTF RLHSNHLFCD CHLAWLSQWL RQRPTIGLFT
   241  QCSGPASLRG LNVAEVQKSE FSCSGQGEAG RVPTCTLSSG SCPAMCTCSN GIVDCRGKGL
   301  TAIPANLPET MTEIRLELNG IKSIPPGAFS PYRKLRRIDL SNNQIAEIAP DAFQGLRSLN
   361  SLVLYGNKIT DLPRGVFGGL YTLQLLLLNA NKINCIRPDA FQDLQNLSLL SLYDNKIQSL
   421  AKGTFTSLRA IQTLHLAQNP FICDCNLKWL ADFLRTNPIE TSGARCASPR RLANKRIGQI
   481  KSKKFRCSAK EQYFIPGTED YQLNSECNSD VVCPHKCRCE ANVVECSSLK LTKIPERIPQ
   541  STAELRLNNN EISILEATGM FKKLTHLKKI NLSNNKVSEI EDGAFEGAAS VSELHLTANQ
   601  LESIRSGMFR GLDGLRTLML RNNRISCIHN DSFTGLRNVR LLSLYDNQIT TVSPGAFDTL
   661  QSLSTLNLLA NPFNCNCQLA WLGGWLRKRK IVTGNPRCQN PDFLRQIPLQ DVAFPDFRCE
   721  EGQEEGGCLP RPQCPQECAC LDTVVRCSNK HLRALPKGIP KNVTELYLDG NQFTLVPGQL
   781  STFKYLQLVD LSNNKISSLS NSSFTNMSQL TTLILSYNAL QCIPPLAFQG LRSLRLLSLH
   841  GNDISTLQEG IFADVTSLSH LAIGANPLYC DCHLRWLSSW VKTGYKEPGI ARCAGPQDME
   901  GKLLLTTPAK KFECQGPPTL AVQAKCDLCL SSPCQNQGTC HNDPLEVYRC ACPSGYKGRD
   961  CEVSLDSCSS GPCENGGTCH AQEGEDAPFT CSCPTGFEGP TCGVNTDDCV DHACANGGVC
  1021  VDGVGNYTCQ CPLQYEGKAC EQLVDLCSPD LNPCQHEAQC VGTPDGPRCE CMPGYAGDNC
  1081  SENQDDCRDH RCQNGAQCMD EVNSYSCLCA EGYSGQLCEI PPHLPAPKSP CEGTECQNGA
  1141  NCVDQGNRPV CQCLPGFGGP ECEKLLSVNF VDRDTYLQFT DLQNWPRANI TLQVSTAEDN
  1201  GILLYNGDND HIAVELYQGH VRVSYDPGSY PSSAIYSAET INDGQFHTVE LVAFDQMVNL
  1261  SIDGGSPMTM DNFGKHYTLN SEAPLYVGGM PVDVNSAAFR LWQILNGTGF HGCIRNLYIN
  1321  NELQDFTKTQ MKPGVVPGCE PCRKLYCLHG ICQPNATPGP MCHCEAGWVG LHCDQPADGP
  1381  CHGHKCVHGQ CVPLDALSYS CQCQDGYSGA LCNQAGALAE PCRGLQCLHG HCQASGTKGA
  1441  HCVCDPGFSG ELCEQESECR GDPVRDFHQV QRGYAICQTT RPLSWVECRG SCPGQGCCQG
  1501  LRLKRRKFTF ECSDGTSFAE EVEKPTKCGC ALCA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SLIT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Secreted

Secreted

Yes

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.31

Highest tissue expression

33 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about SLIT1.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 212 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Monomelic amyotrophy

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.22

gnomAD pLI

1

gnomAD missense Z

3.09

DepMap mean gene effect

0.06

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• axon extension involved in axon guidance
• axon guidance
• dorsal/ventral axon guidance
• forebrain morphogenesis
• motor neuron axon guidance
• negative chemotaxis
• negative regulation of axon extension involved in axon guidance
• negative regulation of synapse assembly
• nuclear migration
• retinal ganglion cell axon guidance
• spinal cord development
• tangential migration from the subventricular zone to the olfactory bulb

Molecular functions

• calcium ion binding
• heparan sulfate proteoglycan binding
• heparin binding
• Roundabout binding

Cellular components

• extracellular space

Protein domainsUniProt · Pfam · InterPro

• EGF-type aspartate/asparagine hydroxylation site
• Leucine-rich repeat N-terminal domain
• Cysteine-rich flanking region, C-terminal
• EGF-like domain
• Leucine-rich repeat
• Laminin G domain
• EGF-like calcium-binding domain
• Leucine-rich repeat, typical subtype
• Follistatin-like, N-terminal
• Cystine knot, C-terminal
• Growth factor receptor cysteine-rich domain superfamily
• EGF-like, conserved site
• Concanavalin A-like lectin/glucanase domain superfamily
• EGF-like calcium-binding, conserved site
• Leucine-rich repeat domain superfamily
• Notch and Slit guidance protein
• EGF-like domain
• Leucine rich repeat N-terminal domain
• Leucine rich repeat C-terminal domain
• Laminin G domain
• Human growth factor-like EGF
• Leucine rich repeat

KeywordsUniProt

• Developmental protein
• Differentiation
• Disulfide bond
• EGF-like domain
• Glycoprotein
• Leucine-rich repeat
• Neurogenesis
• Repeat
• Secreted
• Signal

InteractionsUniProt · HPA

Protein binding partners of SLIT1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• GREM1
• ROBO1