RPL35A

Protein identityUniProt · HPA

UniProt accession

P18077

Gene

RPL35A

Ensembl

ENSG00000182899

Chromosome

3

Canonical length

110 aa

Protein class

Disease related genes, Human disease related genes, Predicted intracellular proteins, Ribosomal proteins

OverviewNCBI Gene

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Alternative splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Oct 2015]

Canonical amino-acid sequenceUniProt

110 residues, UniProt reviewed canonical sequence.

>P18077|RPL35A
     1  MSGRLWSKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP
    61  GGKPNKTRVI WGKVTRAHGN SGMVRAKFRS NLPAKAIGHR IRVMLYPSRI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPL35A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.35

Highest tissue expression

2,292 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RPL35A.

Disease | AllUniProt

Conditions RPL35A is implicated in, by any mechanism.

• Diamond-Blackfan anemia 5 (DBA5) MIM:612528

Disease | GeneticClinVar

13 pathogenic / likely-pathogenic of 144 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Diamond-Blackfan anemia 5
• Diamond-Blackfan anemia

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.41

gnomAD pLI

0.9

gnomAD missense Z

1.37

DepMap mean gene effect

-1.56

DepMap dependency class

pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cytoplasmic translation
• ribosomal large subunit biogenesis
• rRNA processing
• translation

Molecular functions

• RNA binding
• structural constituent of ribosome
• tRNA binding

Cellular components

• cytoplasm
• cytosol
• cytosolic large ribosomal subunit
• cytosolic ribosome
• extracellular exosome
• membrane
• synapse

Protein domainsUniProt · Pfam · InterPro

• Translation protein, beta-barrel domain superfamily
• Large ribosomal subunit protein eL33
• Large ribosomal subunit protein eL33, conserved site
• Large ribosomal subunit protein eL33 superfamily
• Ribosomal protein L35Ae

KeywordsUniProt

• Acetylation
• Cytoplasm
• Diamond-Blackfan anemia
• Ribonucleoprotein
• Ribosomal protein
• RNA-binding
• tRNA-binding

InteractionsUniProt · HPA

Protein binding partners of RPL35A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• RPL5
• RPS16
• SH3GL3
• SRP68