PLXNA4

Protein identityUniProt · HPA

UniProt accession

Q9HCM2

Gene

PLXNA4

Ensembl

ENSG00000221866

Chromosome

7

Canonical length

1894 aa

Protein class

Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Plasma membrane

Secretome location

Intracellular and membrane

OverviewNCBI Gene

Predicted to enable semaphorin receptor activity. Predicted to be involved in nervous system development; regulation of cell migration; and semaphorin-plexin signaling pathway. Predicted to act upstream of or within nervous system development; regulation of axon extension involved in axon guidance; and regulation of negative chemotaxis. Predicted to be located in membrane. Predicted to be part of semaphorin receptor complex. Predicted to be active in cerebellar climbing fiber to Purkinje cell synapse and plasma membrane. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

1894 residues, UniProt reviewed canonical sequence.

>Q9HCM2|PLXNA4
     1  MKAMPWNWTC LLSHLLMVGM GSSTLLTRQP APLSQKQRSF VTFRGEPAEG FNHLVVDERT
    61  GHIYLGAVNR IYKLSSDLKV LVTHETGPDE DNPKCYPPRI VQTCNEPLTT TNNVNKMLLI
   121  DYKENRLIAC GSLYQGICKL LRLEDLFKLG EPYHKKEHYL SGVNESGSVF GVIVSYSNLD
   181  DKLFIATAVD GKPEYFPTIS SRKLTKNSEA DGMFAYVFHD EFVASMIKIP SDTFTIIPDF
   241  DIYYVYGFSS GNFVYFLTLQ PEMVSPPGST TKEQVYTSKL VRLCKEDTAF NSYVEVPIGC
   301  ERSGVEYRLL QAAYLSKAGA VLGRTLGVHP DDDLLFTVFS KGQKRKMKSL DESALCIFIL
   361  KQINDRIKER LQSCYRGEGT LDLAWLKVKD IPCSSALLTI DDNFCGLDMN APLGVSDMVR
   421  GIPVFTEDRD RMTSVIAYVY KNHSLAFVGT KSGKLKKIRV DGPRGNALQY ETVQVVDPGP
   481  VLRDMAFSKD HEQLYIMSER QLTRVPVESC GQYQSCGECL GSGDPHCGWC VLHNTCTRKE
   541  RCERSKEPRR FASEMKQCVR LTVHPNNISV SQYNVLLVLE TYNVPELSAG VNCTFEDLSE
   601  MDGLVVGNQI QCYSPAAKEV PRIITENGDH HVVQLQLKSK ETGMTFASTS FVFYNCSVHN
   661  SCLSCVESPY RCHWCKYRHV CTHDPKTCSF QEGRVKLPED CPQLLRVDKI LVPVEVIKPI
   721  TLKAKNLPQP QSGQRGYECI LNIQGSEQRV PALRFNSSSV QCQNTSYSYE GMEINNLPVE
   781  LTVVWNGHFN IDNPAQNKVH LYKCGAMRES CGLCLKADPD FACGWCQGPG QCTLRQHCPA
   841  QESQWLELSG AKSKCTNPRI TEIIPVTGPR EGGTKVTIRG ENLGLEFRDI ASHVKVAGVE
   901  CSPLVDGYIP AEQIVCEMGE AKPSQHAGFV EICVAVCRPE FMARSSQLYY FMTLTLSDLK
   961  PSRGPMSGGT QVTITGTNLN AGSNVVVMFG KQPCLFHRRS PSYIVCNTTS SDEVLEMKVS
  1021  VQVDRAKIHQ DLVFQYVEDP TIVRIEPEWS IVSGNTPIAV WGTHLDLIQN PQIRAKHGGK
  1081  EHINICEVLN ATEMTCQAPA LALGPDHQSD LTERPEEFGF ILDNVQSLLI LNKTNFTYYP
  1141  NPVFEAFGPS GILELKPGTP IILKGKNLIP PVAGGNVKLN YTVLVGEKPC TVTVSDVQLL
  1201  CESPNLIGRH KVMARVGGME YSPGMVYIAP DSPLSLPAIV SIAVAGGLLI IFIVAVLIAY
  1261  KRKSRESDLT LKRLQMQMDN LESRVALECK EAFAELQTDI HELTSDLDGA GIPFLDYRTY
  1321  TMRVLFPGIE DHPVLRDLEV PGYRQERVEK GLKLFAQLIN NKVFLLSFIR TLESQRSFSM
  1381  RDRGNVASLI MTVLQSKLEY ATDVLKQLLA DLIDKNLESK NHPKLLLRRT ESVAEKMLTN
  1441  WFTFLLYKFL KECAGEPLFS LFCAIKQQME KGPIDAITGE ARYSLSEDKL IRQQIDYKTL
  1501  VLSCVSPDNA NSPEVPVKIL NCDTITQVKE KILDAIFKNV PCSHRPKAAD MDLEWRQGSG
  1561  ARMILQDEDI TTKIENDWKR LNTLAHYQVP DGSVVALVSK QVTAYNAVNN STVSRTSASK
  1621  YENMIRYTGS PDSLRSRTPM ITPDLESGVK MWHLVKNHEH GDQKEGDRGS KMVSEIYLTR
  1681  LLATKGTLQK FVDDLFETIF STAHRGSALP LAIKYMFDFL DEQADKHGIH DPHVRHTWKS
  1741  NCLPLRFWVN MIKNPQFVFD IHKNSITDAC LSVVAQTFMD SCSTSEHRLG KDSPSNKLLY
  1801  AKDIPSYKNW VERYYSDIGK MPAISDQDMN AYLAEQSRMH MNEFNTMSAL SEIFSYVGKY
  1861  SEEILGPLDH DDQCGKQKLA YKLEQVITLM SLDS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PLXNA4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.28

Highest tissue expression

13 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about PLXNA4.

Disease | ImmuneIEDB

Conditions an epitope on PLXNA4 was assayed in.

• berylliosis T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.23

gnomAD pLI

1

gnomAD missense Z

3.8

DepMap mean gene effect

0.08

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• anterior commissure morphogenesis
• axon guidance
• branchiomotor neuron axon guidance
• embryonic heart tube development
• facial nerve structural organization
• glossopharyngeal nerve morphogenesis
• maintenance of synapse structure
• motor neuron axon guidance
• postganglionic parasympathetic fiber development
• regulation of axon extension involved in axon guidance
• regulation of cell migration
• semaphorin-plexin signaling pathway
• sympathetic nervous system development
• sympathetic neuron axon guidance
• synapse assembly
• trigeminal nerve structural organization
• vagus nerve morphogenesis
• chemorepulsion of branchiomotor axon
• regulation of negative chemotaxis

Molecular functions

• semaphorin receptor activity

Cellular components

• cerebellar climbing fiber to Purkinje cell synapse
• plasma membrane
• semaphorin receptor complex

Protein domainsUniProt · Pfam · InterPro

• Sema domain
• Plexin repeat
• IPT domain
• Rho GTPase activation protein
• Plexin, cytoplasmic RasGAP domain
• Immunoglobulin-like fold
• Immunoglobulin E-set
• WD40/YVTN repeat-like-containing domain superfamily
• PSI domain
• Plexin family
• Sema domain superfamily
• Plexin, TIG domain 1
• Plexin, TIG domain 2
• Plexin, cytoplasmic RhoGTPase-binding domain
• Sema domain
• Plexin repeat
• IPT/TIG domain
• Plexin cytoplasmic RasGAP domain
• TIG domain
• TIG domain found in plexin
• Plexin cytoplasmic RhoGTPase-binding domain
• Plexin A/B, PSI domain

KeywordsUniProt

• Acetylation
• Cell membrane
• Disulfide bond
• Glycoprotein
• Membrane
• Receptor
• Repeat
• Signal
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of PLXNA4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• NRP1
• NRP2
• MICAL2