PIP4K2A

Protein identityUniProt · HPA

UniProt accession

P48426

Gene

PIP4K2A

Ensembl

ENSG00000150867

Chromosome

10

Canonical length

406 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Golgi apparatus,Plasma membrane,Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

406 residues, UniProt reviewed canonical sequence.

>P48426|PIP4K2A
     1  MATPGNLGSS VLASKTKTKK KHFVAQKVKL FRASDPLLSV LMWGVNHSIN ELSHVQIPVM
    61  LMPDDFKAYS KIKVDNHLFN KENMPSHFKF KEYCPMVFRN LRERFGIDDQ DFQNSLTRSA
   121  PLPNDSQARS GARFHTSYDK RYIIKTITSE DVAEMHNILK KYHQYIVECH GITLLPQFLG
   181  MYRLNVDGVE IYVIVTRNVF SHRLSVYRKY DLKGSTVARE ASDKEKAKEL PTLKDNDFIN
   241  EGQKIYIDDN NKKVFLEKLK KDVEFLAQLK LMDYSLLVGI HDVERAEQEE VECEENDGEE
   301  EGESDGTHPV GTPPDSPGNT LNSSPPLAPG EFDPNIDVYG IKCHENSPRK EVYFMAIIDI
   361  LTHYDAKKKA AHAAKTVKHG AGAEISTVNP EQYSKRFLDF IGHILT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PIP4K2A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.32

Highest tissue expression

239 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.56

gnomAD pLI

0.14

gnomAD missense Z

1.36

DepMap mean gene effect

0.11

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process
• autophagosome-lysosome fusion
• megakaryocyte development
• negative regulation of insulin receptor signaling pathway
• phosphatidylinositol phosphate biosynthetic process
• positive regulation of autophagosome assembly
• regulation of autophagy
• vesicle-mediated cholesterol transport

Molecular functions

• 1-phosphatidylinositol-4-phosphate 5-kinase activity
• 1-phosphatidylinositol-5-phosphate 4-kinase activity
• ATP binding
• protein homodimerization activity

Cellular components

• autophagosome
• cytosol
• lysosome
• nucleoplasm
• photoreceptor inner segment
• photoreceptor outer segment
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Phosphatidylinositol-4-phosphate 4/5-kinase, core
• Phosphatidylinositol-4/5-phosphate 5/4-kinase
• Phosphatidylinositol-4-phosphate 4/5-kinase, C-terminal domain superfamily
• Phosphatidylinositol-4-phosphate 5-kinase, N-terminal
• Phosphatidylinositol-4-phosphate 5-Kinase

KeywordsUniProt

• Acetylation
• ATP-binding
• Cell membrane
• Cell projection
• Cytoplasm
• Kinase
• Lipid metabolism
• Lysosome
• Membrane
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• Transferase

InteractionsUniProt · HPA

Protein binding partners of PIP4K2A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PIP4K2B
• PIP5K1A
• PIP4K2C
• STK11