NUP98

Protein identityUniProt · HPA

UniProt accession

P52948

Gene

NUP98

Ensembl

ENSG00000110713

Chromosome

11

Canonical length

1817 aa

Protein class

Cancer-related genes, Disease related genes, Metabolic proteins, Plasma proteins, Potential drug targets, Predicted intracellular proteins, Predicted membrane proteins, Transporters

OverviewNCBI Gene

Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin. [provided by RefSeq, Feb 2016]

Canonical amino-acid sequenceUniProt

1817 residues, UniProt reviewed canonical sequence.

>P52948|NUP98
     1  MFNKSFGTPF GGGTGGFGTT STFGQNTGFG TTSGGAFGTS AFGSSNNTGG LFGNSQTKPG
    61  GLFGTSSFSQ PATSTSTGFG FGTSTGTANT LFGTASTGTS LFSSQNNAFA QNKPTGFGNF
   121  GTSTSSGGLF GTTNTTSNPF GSTSGSLFGP SSFTAAPTGT TIKFNPPTGT DTMVKAGVST
   181  NISTKHQCIT AMKEYESKSL EELRLEDYQA NRKGPQNQVG AGTTTGLFGS SPATSSATGL
   241  FSSSTTNSGF AYGQNKTAFG TSTTGFGTNP GGLFGQQNQQ TTSLFSKPFG QATTTQNTGF
   301  SFGNTSTIGQ PSTNTMGLFG VTQASQPGGL FGTATNTSTG TAFGTGTGLF GQTNTGFGAV
   361  GSTLFGNNKL TTFGSSTTSA PSFGTTSGGL FGNKPTLTLG TNTNTSNFGF GTNTSGNSIF
   421  GSKPAPGTLG TGLGAGFGTA LGAGQASLFG NNQPKIGGPL GTGAFGAPGF NTTTATLGFG
   481  APQAPVALTD PNASAAQQAV LQQHINSLTY SPFGDSPLFR NPMSDPKKKE ERLKPTNPAA
   541  QKALTTPTHY KLTPRPATRV RPKALQTTGT AKSHLFDGLD DDEPSLANGA FMPKKSIKKL
   601  VLKNLNNSNL FSPVNRDSEN LASPSEYPEN GERFSFLSKP VDENHQQDGD EDSLVSHFYT
   661  NPIAKPIPQT PESAGNKHSN SNSVDDTIVA LNMRAALRNG LEGSSEETSF HDESLQDDRE
   721  EIENNSYHMH PAGIILTKVG YYTIPSMDDL AKITNEKGEC IVSDFTIGRK GYGSIYFEGD
   781  VNLTNLNLDD IVHIRRKEVV VYLDDNQKPP VGEGLNRKAE VTLDGVWPTD KTSRCLIKSP
   841  DRLADINYEG RLEAVSRKQG AQFKEYRPET GSWVFKVSHF SKYGLQDSDE EEEEHPSKTS
   901  TKKLKTAPLP PASQTTPLQM ALNGKPAPPP QSQSPEVEQL GRVVELDSDM VDITQEPVLD
   961  TMLEESMPED QEPVSASTHI ASSLGINPHV LQIMKASLLT DEEDVDMALD QRFSRLPSKA
  1021  DTSQEICSPR LPISASHSSK TRSLVGGLLQ SKFTSGAFLS PSVSVQECRT PRAASLMNIP
  1081  STSSWSVPPP LTSVFTMPSP APEVPLKTVG TRRQLGLVPR EKSVTYGKGK LLMDMALFMG
  1141  RSFRVGWGPN WTLANSGEQL NGSHELENHQ IADSMEFGFL PNPVAVKPLT ESPFKVHLEK
  1201  LSLRQRKPDE DMKLYQTPLE LKLKHSTVHV DELCPLIVPN LGVAVIHDYA DWVKEASGDL
  1261  PEAQIVKHWS LTWTLCEALW GHLKELDSQL NEPREYIQIL ERRRAFSRWL SCTATPQIEE
  1321  EVSLTQKNSP VEAVFSYLTG KRISEACSLA QQSGDHRLAL LLSQFVGSQS VRELLTMQLV
  1381  DWHQLQADSF IQDERLRIFA LLAGKPVWQL SEKKQINVCS QLDWKRSLAI HLWYLLPPTA
  1441  SISRALSMYE EAFQNTSDSD RYACSPLPSY LEGSGCVIAE EQNSQTPLRD VCFHLLKLYS
  1501  DRHYDLNQLL EPRSITADPL DYRLSWHLWE VLRALNYTHL SAQCEGVLQA SYAGQLESEG
  1561  LWEWAIFVLL HIDNSGIREK AVRELLTRHC QLLETPESWA KETFLTQKLR VPAKWIHEAK
  1621  AVRAHMESDK HLEALCLFKA EHWNRCHKLI IRHLASDAII NENYDYLKGF LEDLAPPERS
  1681  SLIQDWETSG LVYLDYIRVI EMLRHIQQVD CSGNDLEQLH IKVTSLCSRI EQIQCYSAKD
  1741  RLAQSDMAKR VANLLRVVLS LHHPPDRTSD STPDPQRVPL RLLAPHIGRL PMPEDYAMDE
  1801  LRSLTQSYLR ELAVGSL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NUP98 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.5

Highest tissue expression

67 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NUP98.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 291 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Hirschsprung disease, susceptibility to, 1

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.12

gnomAD pLI

1

gnomAD missense Z

1.41

DepMap mean gene effect

-0.92

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• mRNA transport
• nuclear pore complex assembly
• nuclear pore organization
• nucleocytoplasmic transport
• positive regulation of mRNA splicing, via spliceosome
• post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery
• protein import into nucleus
• proteolysis
• RNA export from nucleus
• telomere tethering at nuclear periphery

Molecular functions

• molecular condensate scaffold activity
• mRNA binding
• nuclear localization sequence binding
• promoter-specific chromatin binding
• RNA binding
• serine-type peptidase activity
• structural constituent of nuclear pore
• transcription coactivator activity

Cellular components

• cytosol
• nuclear body
• nuclear envelope
• nuclear inclusion body
• nuclear membrane
• nuclear periphery
• nuclear pore
• nuclear pore cytoplasmic filaments
• nuclear pore nuclear basket
• nuclear pore outer ring
• nucleoplasm
• ribonucleoprotein complex

Protein domainsUniProt · Pfam · InterPro

• Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain
• Nuclear pore complex protein NUP96, C-terminal domain
• Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain superfamily
• Nucleoporin peptidase S59-like
• Nup98-96 autopeptidase S59
• Nuclear protein 96
• Nup98, Gle2-binding sequence

KeywordsUniProt

• Acetylation
• Autocatalytic cleavage
• Chromosomal rearrangement
• Host-virus interaction
• Hydrolase
• Isopeptide bond
• Membrane
• mRNA transport
• Nuclear pore complex
• Nucleus
• Phosphoprotein
• Protease
• Protein transport
• Repeat
• Serine protease
• Translocation
• Transport
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of NUP98 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• NUP214
• NUP88
• NUP96
• RAE1
• NUMA1
• NUP107
• NUP133
• NXF1
• SEC13
• XPO1