NR5A2

Protein identityUniProt · HPA

UniProt accession

O00482

Gene

NR5A2

Ensembl

ENSG00000116833

Chromosome

1

Canonical length

541 aa

Protein class

Nuclear receptors, Predicted intracellular proteins, Transcription factors, Transporters

Subcellular location

Nuclear speckles

OverviewNCBI Gene

The protein encoded by this gene is a DNA-binding zinc finger transcription factor and is a member of the fushi tarazu factor-1 subfamily of orphan nuclear receptors. The encoded protein is involved in the expression of genes for hepatitis B virus and cholesterol biosynthesis, and may be an important regulator of embryonic development. [provided by RefSeq, Jun 2016]

Canonical amino-acid sequenceUniProt

541 residues, UniProt reviewed canonical sequence.

>O00482|NR5A2
     1  MSSNSDTGDL QESLKHGLTP IGAGLPDRHG SPIPARGRLV MLPKVETEAL GLARSHGEQG
    61  QMPENMQVSQ FKMVNYSYDE DLEELCPVCG DKVSGYHYGL LTCESCKGFF KRTVQNNKRY
   121  TCIENQNCQI DKTQRKRCPY CRFQKCLSVG MKLEAVRADR MRGGRNKFGP MYKRDRALKQ
   181  QKKALIRANG LKLEAMSQVI QAMPSDLTIS SAIQNIHSAS KGLPLNHAAL PPTDYDRSPF
   241  VTSPISMTMP PHGSLQGYQT YGHFPSRAIK SEYPDPYTSS PESIMGYSYM DSYQTSSPAS
   301  IPHLILELLK CEPDEPQVQA KIMAYLQQEQ ANRSKHEKLS TFGLMCKMAD QTLFSIVEWA
   361  RSSIFFRELK VDDQMKLLQN CWSELLILDH IYRQVVHGKE GSIFLVTGQQ VDYSIIASQA
   421  GATLNNLMSH AQELVAKLRS LQFDQREFVC LKFLVLFSLD VKNLENFQLV EGVQEQVNAA
   481  LLDYTMCNYP QQTEKFGQLL LRLPEIRAIS MQAEEYLYYK HLNGDVPYNN LLIEMLHAKR
   541  A

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NR5A2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.44

Highest tissue expression

58 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.45

gnomAD pLI

0.24

gnomAD missense Z

1.38

DepMap mean gene effect

-0.01

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• acinar cell differentiation
• bile acid metabolic process
• calcineurin-mediated signaling
• cartilage development
• cellular response to leukemia inhibitory factor
• cholesterol homeostasis
• chromatin remodeling
• embryo development ending in birth or egg hatching
• embryonic cleavage
• exocrine pancreas development
• homeostatic process
• hormone-mediated signaling pathway
• inner cell mass cell differentiation
• morula formation
• negative regulation of chondrocyte differentiation
• negative regulation of inflammatory response
• neurogenesis
• pancreas morphogenesis
• positive regulation of DNA-templated transcription
• positive regulation of stem cell differentiation
• positive regulation of T cell activation
• positive regulation of T cell anergy
• positive regulation of T cell proliferation
• positive regulation of tendon cell differentiation
• positive regulation of transcription by RNA polymerase II
• positive regulation of viral genome replication
• primary ovarian follicle growth
• regulation of DNA-templated transcription
• regulation of transcription by RNA polymerase II
• Sertoli cell development
• somatic stem cell population maintenance
• spermatogenesis
• tissue development
• positive regulation of glucocorticoid biosynthetic process
• zygotic genome activation

Molecular functions

• chromatin binding
• DNA binding
• DNA-binding transcription activator activity, RNA polymerase II-specific
• DNA-binding transcription factor activity
• DNA-binding transcription factor activity, RNA polymerase II-specific
• nuclear receptor activity
• phospholipid binding
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• sequence-specific DNA binding
• sequence-specific double-stranded DNA binding
• transcription cis-regulatory region binding
• transcription coregulator binding
• zinc ion binding

Cellular components

• chromatin
• cytoplasm
• nucleoplasm
• nucleus
• RNA polymerase II transcription regulator complex

Protein domainsUniProt · Pfam · InterPro

• Nuclear hormone receptor, ligand-binding domain
• Zinc finger, nuclear hormone receptor-type
• Nuclear hormone receptor
• Zinc finger, NHR/GATA-type
• Nuclear hormone receptor family 5-like
• Nuclear hormone receptor-like domain superfamily
• Ligand-binding domain of nuclear hormone receptor
• Double treble clef zinc finger, C4 type

KeywordsUniProt

• Activator
• Chromosome
• Developmental protein
• DNA-binding
• Isopeptide bond
• Lipid-binding
• Metal-binding
• Nucleus
• Receptor
• Transcription
• Transcription regulation
• Ubl conjugation
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of NR5A2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• GRIP1
• HNF1A
• NCOA2
• NR0B2
• PPARGC1A
• CTNNB1
• NR0B1