Seroatlas · Human Serome Atlas

NPLOC4

Nuclear protein localization protein 4 homolog

Also known as: FLJ20657, KIAA1499, NPL4, NPL4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8TAT6
Gene: NPLOC4
Ensembl: ENSG00000182446
Chromosome: 17
Canonical length: 608 aa
Protein class: Predicted intracellular proteins, Transporters
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

Predicted to enable ubiquitin binding activity and ubiquitin protein ligase binding activity. Predicted to contribute to K48-linked polyubiquitin modification-dependent protein binding activity and K63-linked polyubiquitin modification-dependent protein binding activity. Involved in negative regulation of RIG-I signaling pathway; negative regulation of type I interferon production; and proteolysis involved in protein catabolic process. Located in nucleus. Part of UFD1-NPL4 complex and VCP-NPL4-UFD1 AAA ATPase complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

608 residues, UniProt reviewed canonical sequence.

>Q8TAT6|NPLOC4
     1  MAESIIIRVQ SPDGVKRITA TKRETAATFL KKVAKEFGFQ NNGFSVYINR NKTGEITASS
    61  NKSLNLLKIK HGDLLFLFPS SLAGPSSEME TSVPPGFKVF GAPNVVEDEI DQYLSKQDGK
   121  IYRSRDPQLC RHGPLGKCVH CVPLEPFDED YLNHLEPPVK HMSFHAYIRK LTGGADKGKF
   181  VALENISCKI KSGCEGHLPW PNGICTKCQP SAITLNRQKY RHVDNIMFEN HTVADRFLDF
   241  WRKTGNQHFG YLYGRYTEHK DIPLGIRAEV AAIYEPPQIG TQNSLELLED PKAEVVDEIA
   301  AKLGLRKVGW IFTDLVSEDT RKGTVRYSRN KDTYFLSSEE CITAGDFQNK HPNMCRLSPD
   361  GHFGSKFVTA VATGGPDNQV HFEGYQVSNQ CMALVRDECL LPCKDAPELG YAKESSSEQY
   421  VPDVFYKDVD KFGNEITQLA RPLPVEYLII DITTTFPKDP VYTFSISQNP FPIENRDVLG
   481  ETQDFHSLAT YLSQNTSSVF LDTISDFHLL LFLVTNEVMP LQDSISLLLE AVRTRNEELA
   541  QTWKRSEQWA TIEQLCSTVG GQLPGLHEYG AVGGSTHTAT AAMWACQHCT FMNQPGTGHC
   601  EMCSLPRT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NPLOC4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.31
Highest tissue expression: 156 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 156 nTPM
heart muscle: 56 nTPM
liver: 53 nTPM
tongue: 52 nTPM
esophagus: 50 nTPM
blood vessel: 47 nTPM

Single-cell type

myonuclei: 222 nCPM
esophageal apical cells: 194 nCPM
retinal ganglion cells: 170 nCPM
proximal tubule cells: 160 nCPM
endometrial glandular cells: 159 nCPM
retinal pigment epithelial cells: 154 nCPM

Immune cell

classical monocyte: 20 nTPM
total PBMC: 9.9 nTPM
myeloid DC: 6.3 nTPM
neutrophil: 5.3 nTPM
intermediate monocyte: 4.8 nTPM
plasmacytoid DC: 4.7 nTPM

Brain region

medulla oblongata: 79 nTPM
hypothalamus: 78 nTPM
cerebral cortex: 77 nTPM
white matter: 76 nTPM
pons: 74 nTPM
thalamus: 73 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.13
gnomAD pLI: 1
gnomAD missense Z: 3.2
DepMap mean gene effect: -1.39
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Zinc finger, RanBP2-type
Ubiquitin-like domain superfamily
Zinc finger, RanBP2-type superfamily
MPN domain
NPL4, zinc-binding putative
Nuclear pore localisation protein NPL4, C-terminal
Nuclear protein localization protein 4
Nuclear pore localisation protein Npl4, ubiquitin-like domain
NPL4 family, putative zinc binding region
NPL4 family
Nuclear pore localisation protein NPL4

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NPLOC4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NPLOC4 as an antibody target. Whether an autoantibody or antibody against NPLOC4 could matter depends on whether native NPLOC4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NPLOC4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NPLOC4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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