NFIL3
Nuclear factor interleukin-3-regulated protein
Also known as: E4BP4, IL3BP1, NF-IL3A, NFIL3_HUMAN, NFIL3A
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q16649
- Gene
- NFIL3
- Ensembl
- ENSG00000165030
- Chromosome
- 9
- Canonical length
- 462 aa
- Protein class
- Predicted intracellular proteins, Transcription factors
- Subcellular location
- Nuclear bodies
- Quaternary structure
- Homodimer
OverviewNCBI Gene
The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]
Canonical amino-acid sequenceUniProt
462 residues, UniProt reviewed canonical sequence.
>Q16649|NFIL3
1 MQLRKMQTVK KEQASLDASS NVDKMMVLNS ALTEVSEDST TGEELLLSEG SVGKNKSSAC
61 RRKREFIPDE KKDAMYWEKR RKNNEAAKRS REKRRLNDLV LENKLIALGE ENATLKAELL
121 SLKLKFGLIS STAYAQEIQK LSNSTAVYFQ DYQTSKSNVS SFVDEHEPSM VSSSCISVIK
181 HSPQSSLSDV SEVSSVEHTQ ESSVQGSCRS PENKFQIIKQ EPMELESYTR EPRDDRGSYT
241 ASIYQNYMGN SFSGYSHSPP LLQVNRSSSN SPRTSETDDG VVGKSSDGED EQQVPKGPIH
301 SPVELKHVHA TVVKVPEVNS SALPHKLRIK AKAMQIKVEA FDNEFEATQK LSSPIDMTSK
361 RHFELEKHSA PSMVHSSLTP FSVQVTNIQD WSLKSEHWHQ KELSGKTQNS FKTGVVEMKD
421 SGYKVSDPEN LYLKQGIANL SAEVVSLKRL IATQPISASD SGLocalizationUniProt · AlphaFold · HPA
Whether an antibody against NFIL3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.65
- Highest tissue expression
- 200 nTPM
Expression across tissuesHPA
Tissue
- bone marrow: 200 nTPM
- adipose tissue: 144 nTPM
- blood vessel: 136 nTPM
- skeletal muscle: 131 nTPM
- liver: 117 nTPM
- ovary: 88 nTPM
Single-cell type
- neutrophils: 1,247 nCPM
- monocytes: 320 nCPM
- smooth muscle cells: 286 nCPM
- vascular smooth muscle cells: 255 nCPM
- endometrial luminal cells: 231 nCPM
- neutrophil progenitors: 194 nCPM
Immune cell
- basophil: 58 nTPM
- neutrophil: 27 nTPM
- eosinophil: 10 nTPM
- classical monocyte: 1.6 nTPM
- gdT-cell: 1.2 nTPM
- non-classical monocyte: 1 nTPM
Brain region
- cerebellum: 19 nTPM
- medulla oblongata: 18 nTPM
- cerebral cortex: 16 nTPM
- pons: 16 nTPM
- midbrain: 15 nTPM
- choroid plexus: 15 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.44
- gnomAD pLI
- 0.82
- gnomAD missense Z
- 1.6
- DepMap mean gene effect
- -0.07
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- cellular response to interleukin-4
- circadian rhythm
- immune response
- natural killer cell differentiation
- negative regulation of DNA-templated transcription
- negative regulation of transcription by RNA polymerase II
- positive regulation of DNA-templated transcription
- positive regulation of gene expression
- regulation of DNA-templated transcription
- regulation of transcription by RNA polymerase II
- transcription by RNA polymerase II
Molecular functions
- DNA-binding transcription factor activity
- DNA-binding transcription factor activity, RNA polymerase II-specific
- DNA-binding transcription repressor activity, RNA polymerase II-specific
- identical protein binding
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- RNA polymerase II transcription regulatory region sequence-specific DNA binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Basic-leucine zipper domain
- Basic-leucine zipper domain superfamily
- Nuclear factor interleukin-3-regulated protein-like , bZIP domain
- Nuclear factor interleukin-3-regulated protein-like
- Basic region leucine zipper
- Vertebrate interleukin-3 regulated transcription factor
- Nuclear factor interleukin-3-regulated protein
- Vertebrate interleukin-3 regulated transcription factor
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of NFIL3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads NFIL3 as an antibody target. Whether an autoantibody or antibody against NFIL3 could matter depends on whether native NFIL3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
NFIL3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label NFIL3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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