Seroatlas · Human Serome Atlas

NASP

Nuclear autoantigenic sperm protein

Also known as: DKFZp547F162, FLB7527, FLJ31599, FLJ35510, MGC19722, MGC20372, MGC2297, NASP_HUMAN, PRO1999

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P49321
Gene: NASP
Ensembl: ENSG00000132780
Chromosome: 1
Canonical length: 788 aa
Protein class: Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

788 residues, UniProt reviewed canonical sequence.

>P49321|NASP
     1  MAMESTATAA VAAELVSADK IEDVPAPSTS ADKVESLDVD SEAKKLLGLG QKHLVMGDIP
    61  AAVNAFQEAA SLLGKKYGET ANECGEAFFF YGKSLLELAR MENGVLGNAL EGVHVEEEEG
   121  EKTEDESLVE NNDNIDEEAR EELREQVYDA MGEKEEAKKT EDKSLAKPET DKEQDSEMEK
   181  GGREDMDISK SAEEPQEKVD LTLDWLTETS EEAKGGAAPE GPNEAEVTSG KPEQEVPDAE
   241  EEKSVSGTDV QEECREKGGQ EKQGEVIVSI EEKPKEVSEE QPVVTLEKQG TAVEVEAESL
   301  DPTVKPVDVG GDEPEEKVVT SENEAGKAVL EQLVGQEVPP AEESPEVTTE AAEASAVEAG
   361  SEVSEKPGQE APVLPKDGAV NGPSVVGDQT PIEPQTSIER LTETKDGSGL EEKVRAKLVP
   421  SQEETKLSVE ESEAAGDGVD TKVAQGATEK SPEDKVQIAA NEETQEREEQ MKEGEETEGS
   481  EEDDKENDKT EEMPNDSVLE NKSLQENEEE EIGNLELAWD MLDLAKIIFK RQETKEAQLY
   541  AAQAHLKLGE VSVESENYVQ AVEEFQSCLN LQEQYLEAHD RLLAETHYQL GLAYGYNSQY
   601  DEAVAQFSKS IEVIENRMAV LNEQVKEAEG SSAEYKKEIE ELKELLPEIR EKIEDAKESQ
   661  RSGNVAELAL KATLVESSTS GFTPGGGGSS VSMIASRKPT DGASSSNCVT DISHLVRKKR
   721  KPEEESPRKD DAKKAKQEPE VNGGSGDAVP SGNEVSENME EEAENQAESR AAVEGTVEAG
   781  ATVESTAC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NASP can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.59
Highest tissue expression: 156 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 156 nTPM
testis: 105 nTPM
thymus: 94 nTPM
tonsil: 91 nTPM
lymph node: 86 nTPM
spleen: 81 nTPM

Single-cell type

early primary spermatocytes: 694 nCPM
differentiating spermatogonia: 600 nCPM
erythrocyte progenitors: 485 nCPM
cytotrophoblasts: 433 nCPM
migrating cytotrophoblasts: 340 nCPM
megakaryocyte progenitors: 336 nCPM

Immune cell

naive B-cell: 23 nTPM
T-reg: 22 nTPM
MAIT T-cell: 16 nTPM
memory CD8 T-cell: 16 nTPM
memory B-cell: 16 nTPM
memory CD4 T-cell: 16 nTPM

Brain region

hypothalamus: 44 nTPM
medulla oblongata: 38 nTPM
white matter: 38 nTPM
pons: 34 nTPM
basal ganglia: 32 nTPM
thalamus: 32 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.28
gnomAD pLI: 1
gnomAD missense Z: 0.61
DepMap mean gene effect: -0.52
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Tetratricopeptide-like helical domain superfamily
Tetratricopeptide repeat
Tetratricopeptide repeat
Tetratricopeptide, SHNi-TPR domain
Nuclear autoantigenic sperm protein-like
SHNi-TPR

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NASP in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NASP as an antibody target. Whether an autoantibody or antibody against NASP could matter depends on whether native NASP is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NASP is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NASP as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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