N4BP2

Protein identityUniProt · HPA

UniProt accession

Q86UW6

Gene

N4BP2

Ensembl

ENSG00000078177

Chromosome

4

Canonical length

1770 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm,Plasma membrane,Cytosol

OverviewNCBI Gene

This gene encodes a protein containing a polynucleotide kinase domain (PNK) near the N-terminal region, and a Small MutS Related (Smr) domain near the C-terminal region. The encoded protein can bind to both B-cell leukemia/lymphoma 3 (BCL-3) and neural precursor cell expressed, developmentally downregulated 4, (Nedd4) proteins. This protein binds and hydrolyzes ATP, may function as a 5'-polynucleotide kinase, and has the capacity to be a ubiquitylation substrate. This protein may play a role in transcription-coupled DNA repair or genetic recombination. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Canonical amino-acid sequenceUniProt

1770 residues, UniProt reviewed canonical sequence.

>Q86UW6|N4BP2
     1  MPRRRKNLGG NPFRKTANPK EVVVSSVASR EEPTTTLPSM GETKVDQEEL FTSISEIFSD
    61  LDPDVVYLML SECDFKVENA MDCLLELSAT DTKIEESSSQ SFVASENQVG AAESKIMEKR
   121  PEEESEDSKM DSFLDMQLTE DLDSLIQNAF EKLNSSPDDQ VYSFLPSQDV NSFNDSSEFI
   181  NPDSSNMTPI FSTQNMNLNG ENLENSGSTL SLNPLPSHSV LNESKCFIKD NTLALESNYP
   241  EDSLLSSSLN VASDSIAGCS SLNQKQKELL ESECVEAQFS EAPVDLDASE PQACLNLPGL
   301  DLPGTGGDQK STRVSDVFLP SEGFNFKPHK HPELPTKGKD VSYCPVLAPL PLLLPPPPPP
   361  PMWNPMIPAF DLFQGNHGFV APVVTTAAHW RSVNYTFPPS VISHTSPTKV WRNKDGTSAY
   421  QVQETPVSQV VRKKTSYVGL VLVLLRGLPG SGKSFLARTL QEDNPSGVIL STDDYFYING
   481  QYQFDVKYLG EAHEWNQNRA KEAFEKKISP IIIDNTNLQA WEMKPYVALS QKHKYKVLFR
   541  EPDTWWKFKP KELARRNIHG VSKEKITRML EHYQRFVSVP IIMSSSVPEK IERIELCAYS
   601  CEDRSTSPRD DEDIISEKEE NILSLSLKHL EFTEEKNLDV TKETMLPENV AYLSNADLNK
   661  RRKEISDMNP SIQSALILET PHMYFSDSES KLQATDKSEN EQIEMVAVKG YSKTDTDSSM
   721  ERVSPSTCCS ENNQEDCDLA NSGPLQNEKS SPGEIVEERA TVTKKAFGKQ KSKSTLEKFP
   781  RHELSNFVGD WPVDKTIGQR TKRNRKTEKT SSVQSDKKYN YPQSHKLVNS VSVNTDCVQQ
   841  RGSPHESVED GRKSQCDDAS EPLNSYKYDA YKNIDKNSFN IMGDWPSSDS LAQREHRSRM
   901  PKTGLSEPNL EIGTNDKMNE ISLSTAHEAC WGTSSQKLKT LGSSNLGSSE MLLSEMTCES
   961  QTCLSKKSHG QHTSLPLTFT NSAPTVSGVV EPQTLAECQE QMPKRDPGKE VGMCTQTEPQ
  1021  DFALLWKIEK NKISISDSIK VLTGRLDGFK PKVFNINTKS DVQEAIPYRV MYDKSTFVEE
  1081  SELTSADESE NLNILCKLFG SFSLEALKDL YERCNKDIIW ATSLLLDSET KLCEDTEFEN
  1141  FQKSCDGSQI GPFSLGLNLK EIISQRGTLE NSNSPVPEFS HGIGISNADS QSTCDAERGN
  1201  SEQAEMRAVT PENHESMTSI FPSAAVGLKN NNDILPNSQE ELLYSSKQSF PGILKATTPK
  1261  DMSETEKNLV VTETGDNIHS PSHFSDIFNF VSSTSNLELN EEIYFTDSLE IKRNENFPKD
  1321  YVKFSDEEEF MNEDEKEMKE ILMAGSSLSA GVSGEDKTEI LNPTPAMAKS LTIDCLELAL
  1381  PPELAFQLNE LFGPVGIDSG SLTVEDCVVH IDLNLAKVIH EKWKESVMER QRQEEVSCGK
  1441  FMQDPSLVGH TGLDNPEQKS SQRTGKKLLK TLTASEMLPL LDHWNTQTKK VSLREIMSEE
  1501  IALQEKHNLK RETLMFEKDC ATKLKEKQLF KIFPAINQNF LVDIFKDHNY SLEHTVQFLN
  1561  CVLEGDPVKT VVAQEFVHQN ENVTSHTGQK SKEKKPKKLK ETEETPSELS FQDFEYPDYD
  1621  DYRAEAFLHQ QKRMECYSKA KEAYRIGKKN VATFYAQQGT LHEQKMKEAN HLAAIEIFEK
  1681  VNASLLPQNV LDLHGLHVDE ALEHLMRVLE KKTEEFKQNG GKPYLSVITG RGNHSQGGVA
  1741  RIKPAVIKYL ISHSFRFSEI KPGCLKVMLK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against N4BP2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.56

Highest tissue expression

11 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.61

gnomAD pLI

0

gnomAD missense Z

0.8

DepMap mean gene effect

-0.08

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• non-stop decay
• nuclear-transcribed mRNA catabolic process

Molecular functions

• ATP binding
• ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity
• DNA endonuclease activity
• endonuclease activity
• hydrolase activity
• ubiquitin binding

Cellular components

• cytosol

Protein domainsUniProt · Pfam · InterPro

• Ubiquitin system component CUE
• UBA-like superfamily
• P-loop containing nucleoside triphosphate hydrolase
• AAA domain
• Smr domain
• Domain of unknown function DUF1771
• Smr domain superfamily
• NEDD4-binding protein 2, CUE domain
• Endonuclease/Polynucleotide Kinase Domain-Containing Protein
• NEDD4-binding protein 2, DUF7816
• NEDD4-binding protein 2, DUF7817
• NEDD4-binding protein 2, DUF7818
• Smr domain
• Domain of unknown function (DUF1771)
• Domain of unknown function (DUF7816)
• Domain of unknown function (DUF7817)
• Domain of unknown function (DUF7818)

KeywordsUniProt

• ATP-binding
• Coiled coil
• Cytoplasm
• Hydrolase
• Nucleotide-binding
• Phosphoprotein
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of N4BP2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• BCL3
• CREBBP
• DYNLL1
• DYNLL2