Seroatlas · Human Serome Atlas

MIDN

Midnolin

Also known as: MIDN_HUMAN, Stx

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q504T8
Gene: MIDN
Ensembl: ENSG00000167470
Chromosome: 19
Canonical length: 468 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Enables molecular adaptor activity. Involved in proteasomal ubiquitin-independent protein catabolic process. Located in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

468 residues, UniProt reviewed canonical sequence.

>Q504T8|MIDN
     1  MEPQPGGARS CRRGAPGGAC ELGPAAEAAP MSLAIHSTTG TRYDLAVPPD ETVEGLRKRL
    61  SQRLKVPKER LALLHKDTRL SSGKLQEFGV GDGSKLTLVP TVEAGLMSQA SRPEQSVMQA
   121  LESLTETQVS DFLSGRSPLT LALRVGDHMM FVQLQLAAQH APLQHRHVLA AAAAAAAARG
   181  DPSIASPVSS PCRPVSSAAR VPPVPTSPSP ASPSPITAGS FRSHAASTTC PEQMDCSPTA
   241  SSSASPGAST TSTPGASPAP RSRKPGAVIE SFVNHAPGVF SGTFSGTLHP NCQDSSGRPR
   301  RDIGTILQIL NDLLSATRHY QGMPPSLAQL RCHAQCSPAS PAPDLAPRTT SCEKLTAAPS
   361  ASLLQGQSQI RMCKPPGDRL RQTENRATRC KVERLQLLLQ QKRLRRKARR DARGPYHWSP
   421  SRKAGRSDSS SSGGGGSPSE ASGLGLDFED SVWKPEVNPD IKSEFVVA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MIDN can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.56
Highest tissue expression: 86 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 86 nTPM
bone marrow: 73 nTPM
esophagus: 66 nTPM
pituitary gland: 57 nTPM
skin: 51 nTPM
heart muscle: 47 nTPM

Single-cell type

esophageal apical cells: 773 nCPM
neutrophils: 749 nCPM
epididymal basal cells: 421 nCPM
esophageal suprabasal cells: 384 nCPM
breast secretory cells: 373 nCPM
prostatic club cells: 373 nCPM

Immune cell

neutrophil: 12 nTPM
basophil: 1.6 nTPM
NK-cell: 1.2 nTPM
eosinophil: 1.1 nTPM
non-classical monocyte: 1.1 nTPM
classical monocyte: 1 nTPM

Brain region

medulla oblongata: 90 nTPM
thalamus: 68 nTPM
hypothalamus: 65 nTPM
cerebral cortex: 63 nTPM
pons: 62 nTPM
midbrain: 62 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.61
gnomAD pLI: 0.18
gnomAD missense Z: 0.42
DepMap mean gene effect: -0.15
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MIDN in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MIDN as an antibody target. Whether an autoantibody or antibody against MIDN could matter depends on whether native MIDN is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MIDN is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MIDN as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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