Seroatlas · Human Serome Atlas

MIB1

E3 ubiquitin-protein ligase MIB1

Also known as: DIP-1, KIAA1323, MIB, MIB1_HUMAN, ZZANK2, ZZZ6

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q86YT6
Gene
MIB1
Ensembl
ENSG00000101752
Chromosome
18
Canonical length
1006 aa
Protein class
Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Potential drug targets, Predicted intracellular proteins
Subcellular location
Microtubules,Mitotic spindle,Primary cilium,Centriolar satellite,Basal body

OverviewNCBI Gene

This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). [provided by RefSeq, Jun 2013]

Canonical amino-acid sequenceUniProt

1006 residues, UniProt reviewed canonical sequence.

>Q86YT6|MIB1
     1  MSNSRNNRVM VEGVGARVVR GPDWKWGKQD GGEGHVGTVR SFESPEEVVV VWDNGTAANY
    61  RCSGAYDLRI LDSAPTGIKH DGTMCDTCRQ QPIIGIRWKC AECTNYDLCT VCYHGDKHHL
   121  RHRFYRITTP GSERVLLESR RKSKKITARG IFAGARVVRG VDWQWEDQDG GNGRRGKVTE
   181  IQDWSASSPH SAAYVLWDNG AKNLYRVGFE GMSDLKCVQD AKGGSFYRDH CPVLGEQNGN
   241  RNPGGLQIGD LVNIDLDLEI VQSLQHGHGG WTDGMFETLT TTGTVCGIDE DHDIVVQYPS
   301  GNRWTFNPAV LTKANIVRSG DAAQGAEGGT SQFQVGDLVQ VCYDLERIKL LQRGHGEWAE
   361  AMLPTLGKVG RVQQIYSDSD LKVEVCGTSW TYNPAAVSKV ASAGSAISNA SGERLSQLLK
   421  KLFETQESGD LNEELVKAAA NGDVAKVEDL LKRPDVDVNG QCAGHTAMQA ASQNGHVDIL
   481  KLLLKQNVDV EAEDKDGDRA VHHAAFGDEG AVIEVLHRGS ADLNARNKRR QTPLHIAVNK
   541  GHLQVVKTLL DFGCHPSLQD SEGDTPLHDA ISKKRDDILA VLLEAGADVT ITNNNGFNAL
   601  HHAALRGNPS AMRVLLSKLP RPWIVDEKKD DGYTALHLAA LNNHVEVAEL LVHQGNANLD
   661  IQNVNQQTAL HLAVERQHTQ IVRLLVRAGA KLDIQDKDGD TPLHEALRHH TLSQLRQLQD
   721  MQDVGKVDAA WEPSKNTLIM GLGTQGAEKK SAASIACFLA ANGADLSIRN KKGQSPLDLC
   781  PDPNLCKALA KCHKEKVSGQ VGSRSPSMIS NDSETLEECM VCSDMKRDTL FGPCGHIATC
   841  SLCSPRVKKC LICKEQVQSR TKIEECVVCS DKKAAVLFQP CGHMCACENC ANLMKKCVQC
   901  RAVVERRVPF IMCCGGKSSE DATDDISSGN IPVLQKDKDN TNVNADVQKL QQQLQDIKEQ
   961  TMCPVCLDRL KNMIFLCGHG TCQLCGDRMS ECPICRKAIE RRILLY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MIB1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.31
Highest tissue expression
13 nTPM

Expression across tissuesHPA

Tissue

  • thyroid gland: 13 nTPM
  • endometrium: 12 nTPM
  • blood vessel: 11 nTPM
  • thymus: 11 nTPM
  • ovary: 11 nTPM
  • breast: 11 nTPM

Single-cell type

  • cardiomyocytes: 2,577 nCPM
  • myonuclei: 980 nCPM
  • podocytes: 526 nCPM
  • choroid plexus epithelial cells: 307 nCPM
  • ependymal cells: 260 nCPM
  • gonadotrophs: 233 nCPM

Immune cell

  • basophil: 0.7 nTPM
  • NK-cell: 0.5 nTPM
  • T-reg: 0.4 nTPM
  • classical monocyte: 0.3 nTPM
  • memory CD4 T-cell: 0.3 nTPM
  • neutrophil: 0.3 nTPM

Brain region

  • thalamus: 27 nTPM
  • midbrain: 27 nTPM
  • spinal cord: 27 nTPM
  • hypothalamus: 26 nTPM
  • medulla oblongata: 25 nTPM
  • white matter: 24 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MIB1.

Disease | AllUniProt

Conditions MIB1 is implicated in, by any mechanism.

  • Left ventricular non-compaction 7 (LVNC7) MIM:615092

Disease | GeneticClinVar

28 pathogenic / likely-pathogenic of 675 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Disease | AutoantibodyPubMed

Conditions in which antibodies against MIB1 are reported. Each links to that disease's full target list.

Showing 2 of 5 — disease pages carrying at least 10 antigens.

ReferencesPubMed · IEDB

Publications for MIB1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

32 publications

Show 20 more of 32 total

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.97
gnomAD pLI
0
gnomAD missense Z
3.21
DepMap mean gene effect
-0.26
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MIB1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MIB1 as an antibody target. Whether an autoantibody or antibody against MIB1 could matter depends on whether native MIB1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MIB1 is annotated at the cell surface, where native MIB1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label MIB1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MIB1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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