Seroatlas · Human Serome Atlas

METTL14

N(6)-adenosine-methyltransferase non-catalytic subunit METTL14

Also known as: KIAA1627, MET14_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9HCE5
Gene
METTL14
Ensembl
ENSG00000145388
Chromosome
4
Canonical length
456 aa
Protein class
Cancer-related genes, Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

Enables mRNA binding activity and mRNA m(6)A methyltransferase activity. Involved in mRNA modification; mRNA splicing, via spliceosome; and mRNA stabilization. Located in nucleoplasm. Is active in RNA N6-methyladenosine methyltransferase complex. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

456 residues, UniProt reviewed canonical sequence.

>Q9HCE5|METTL14
     1  MDSRLQEIRE RQKLRRQLLA QQLGAESADS IGAVLNSKDE QREIAETRET CRASYDTSAP
    61  NAKRKYLDEG ETDEDKMEEY KDELEMQQDE ENLPYEEEIY KDSSTFLKGT QSLNPHNDYC
   121  QHFVDTGHRP QNFIRDVGLA DRFEEYPKLR ELIRLKDELI AKSNTPPMYL QADIEAFDIR
   181  ELTPKFDVIL LEPPLEEYYR ETGITANEKC WTWDDIMKLE IDEIAAPRSF IFLWCGSGEG
   241  LDLGRVCLRK WGYRRCEDIC WIKTNKNNPG KTKTLDPKAV FQRTKEHCLM GIKGTVKRST
   301  DGDFIHANVD IDLIITEEPE IGNIEKPVEI FHIIEHFCLG RRRLHLFGRD STIRPGWLTV
   361  GPTLTNSNYN AETYASYFSA PNSYLTGCTE EIERLRPKSP PPKSKSDRGG GAPRGGGRGG
   421  TSAGRGRERN RSNFRGERGG FRGGRGGAHR GGFPPR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against METTL14 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.41
Highest tissue expression
18 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 18 nTPM
  • testis: 14 nTPM
  • liver: 14 nTPM
  • thymus: 12 nTPM
  • endometrium: 11 nTPM
  • breast: 11 nTPM

Single-cell type

  • early spermatids: 134 nCPM
  • early primary spermatocytes: 68 nCPM
  • late primary spermatocytes: 65 nCPM
  • pituicytes/fscs: 57 nCPM
  • sertoli cells: 56 nCPM
  • thyrotrophs: 50 nCPM

Immune cell

  • basophil: 38 nTPM
  • eosinophil: 29 nTPM
  • naive B-cell: 26 nTPM
  • memory B-cell: 25 nTPM
  • non-classical monocyte: 24 nTPM
  • NK-cell: 22 nTPM

Brain region

  • white matter: 16 nTPM
  • cerebellum: 15 nTPM
  • hypothalamus: 15 nTPM
  • medulla oblongata: 14 nTPM
  • basal ganglia: 14 nTPM
  • choroid plexus: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.61
gnomAD pLI
0
gnomAD missense Z
2.74
DepMap mean gene effect
-0.66
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of METTL14 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads METTL14 as an antibody target. Whether an autoantibody or antibody against METTL14 could matter depends on whether native METTL14 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

METTL14 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label METTL14 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/METTL14. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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