MAP2K5

Protein identityUniProt · HPA

UniProt accession

Q13163

Gene

MAP2K5

Ensembl

ENSG00000137764

Chromosome

15

Canonical length

448 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Nucleoli,Microtubules,Cytokinetic bridge,Primary cilium,Basal body

OverviewNCBI Gene

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011]

Canonical amino-acid sequenceUniProt

448 residues, UniProt reviewed canonical sequence.

>Q13163|MAP2K5
     1  MLWLALGPFP AMENQVLVIR IKIPNSGAVD WTVHSGPQLL FRDVLDVIGQ VLPEATTTAF
    61  EYEDEDGDRI TVRSDEEMKA MLSYYYSTVM EQQVNGQLIE PLQIFPRACK PPGERNIHGL
   121  KVNTRAGPSQ HSSPAVSDSL PSNSLKKSSA ELKKILANGQ MNEQDIRYRD TLGHGNGGTV
   181  YKAYHVPSGK ILAVKVILLD ITLELQKQIM SELEILYKCD SSYIIGFYGA FFVENRISIC
   241  TEFMDGGSLD VYRKMPEHVL GRIAVAVVKG LTYLWSLKIL HRDVKPSNML VNTRGQVKLC
   301  DFGVSTQLVN SIAKTYVGTN AYMAPERISG EQYGIHSDVW SLGISFMELA LGRFPYPQIQ
   361  KNQGSLMPLQ LLQCIVDEDS PVLPVGEFSE PFVHFITQCM RKQPKERPAP EELMGHPFIV
   421  QFNDGNAAVV SMWVCRALEE RRSQQGPP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAP2K5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.32

Highest tissue expression

22 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.51

gnomAD pLI

0

gnomAD missense Z

2.02

DepMap mean gene effect

-0.09

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to growth factor stimulus
• cellular response to laminar fluid shear stress
• heart development
• insulin-like growth factor receptor signaling pathway
• MAPK cascade
• negative regulation of canonical NF-kappaB signal transduction
• negative regulation of cell migration involved in sprouting angiogenesis
• negative regulation of chemokine (C-X-C motif) ligand 2 production
• negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
• negative regulation of heterotypic cell-cell adhesion
• negative regulation of interleukin-8 production
• negative regulation of response to cytokine stimulus
• negative regulation of smooth muscle cell apoptotic process
• negative regulation of transcription by RNA polymerase II
• positive regulation of cell growth
• positive regulation of epithelial cell proliferation
• positive regulation of protein metabolic process
• positive regulation of transcription by RNA polymerase II
• signal transduction
• ERK5 cascade

Molecular functions

• ATP binding
• MAP kinase kinase activity
• metal ion binding
• protein kinase activity
• protein serine kinase activity
• protein serine/threonine kinase activity
• protein tyrosine kinase activity

Cellular components

• spindle

Protein domainsUniProt · Pfam · InterPro

• PB1 domain
• Protein kinase domain
• Serine/threonine-protein kinase, active site
• Protein kinase-like domain superfamily
• Protein kinase, ATP binding site
• Dual specificity MAP kinase kinase
• PB1-like domain
• Protein kinase domain
• PB1 domain
• Dual specificity mitogen-activated protein kinase kinase 5, PB1 domain

KeywordsUniProt

• Acetylation
• ATP-binding
• Kinase
• Magnesium
• Metal-binding
• Nucleotide-binding
• Phosphoprotein
• Serine/threonine-protein kinase
• Transferase
• Tyrosine-protein kinase

InteractionsUniProt · HPA

Protein binding partners of MAP2K5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MAP3K3
• MAPK7
• PARD6A
• SQSTM1
• HSP90AB1
• LNX2
• MAP3K2