MAGEA3

Protein identityUniProt · HPA

UniProt accession

P43357

Gene

MAGEA3

Ensembl

ENSG00000221867

Chromosome

X

Canonical length

314 aa

Protein class

Cancer-related genes, Predicted intracellular proteins

OverviewNCBI Gene

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Canonical amino-acid sequenceUniProt

314 residues, UniProt reviewed canonical sequence.

>P43357|MAGEA3
     1  MPLEQRSQHC KPEEGLEARG EALGLVGAQA PATEEQEAAS SSSTLVEVTL GEVPAAESPD
    61  PPQSPQGASS LPTTMNYPLW SQSYEDSSNQ EEEGPSTFPD LESEFQAALS RKVAELVHFL
   121  LLKYRAREPV TKAEMLGSVV GNWQYFFPVI FSKASSSLQL VFGIELMEVD PIGHLYIFAT
   181  CLGLSYDGLL GDNQIMPKAG LLIIVLAIIA REGDCAPEEK IWEELSVLEV FEGREDSILG
   241  DPKKLLTQHF VQENYLEYRQ VPGSDPACYE FLWGPRALVE TSYVKVLHHM VKISGGPHIS
   301  YPPLHEWVLR EGEE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAGEA3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.45

Highest tissue expression

7.6 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MAGEA3.

Disease | ImmuneIEDB

Conditions an epitope on MAGEA3 was assayed in.

• skin melanoma B and T cell
• melanoma T cell
• hepatocellular carcinoma T cell
• lung cancer T cell
• lung non-small cell carcinoma T cell
• testis seminoma T cell
• pancreatic adenocarcinoma T cell
• stomach cancer B cell
• gastrointestinal system disease B cell
• cholangiocarcinoma T cell
• multiple myeloma T cell
• anaplastic oligodendroglioma T cell
• hepatitis C T cell
• ovarian cancer T cell
• liver cirrhosis T cell
• testicular germ cell cancer T cell
• smoldering myeloma T cell
• lung adenocarcinoma T cell
• brain glioblastoma multiforme T cell
• castration-resistant prostate carcinoma T cell

ReferencesPubMed · IEDB

Publications for MAGEA3 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD missense Z

-2.34

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• negative regulation of autophagy
• negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
• negative regulation of protein processing
• negative regulation of transcription by RNA polymerase II

Molecular functions

• caspase binding
• histone deacetylase binding

Cellular components

• endoplasmic reticulum
• nucleus

Protein domainsUniProt · Pfam · InterPro

• MAGE homology domain
• Melanoma associated antigen, N-terminal
• Melanoma-associated antigen
• MAGE homology domain, winged helix WH1 motif
• MAGE homology domain, winged helix WH2 motif
• MAGE homology domain
• Melanoma associated antigen family N terminal

KeywordsUniProt

• Tumor antigen
• Ubl conjugation
• Ubl conjugation pathway

InteractionsUniProt · HPA

Protein binding partners of MAGEA3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TRIM28
• BACC1
• STAMBPL1