LDHB

Protein identityUniProt · HPA

UniProt accession

P07195

Gene

LDHB

Ensembl

ENSG00000111716

Chromosome

12

Canonical length

334 aa

Protein class

Disease related genes, Enzymes, FDA approved drug targets, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Cytosol

Quaternary structure

Homotetramer

OverviewNCBI Gene

This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016]

Canonical amino-acid sequenceUniProt

334 residues, UniProt reviewed canonical sequence.

>P07195|LDHB
     1  MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK
    61  GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF
   121  IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG
   181  IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY
   241  EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG
   301  LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against LDHB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

1,825 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about LDHB.

Disease | AllUniProt

Conditions LDHB is implicated in, by any mechanism.

• Lactate dehydrogenase B deficiency (LDHBD) MIM:614128

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.2

gnomAD pLI

0

gnomAD missense Z

0.91

DepMap mean gene effect

0.02

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• lactate metabolic process
• NAD+ metabolic process
• pyruvate catabolic process

Molecular functions

• identical protein binding
• kinase binding
• L-lactate dehydrogenase (NAD+) activity
• NAD binding

Cellular components

• cytoplasm
• cytosol
• extracellular exosome
• membrane
• membrane raft
• mitochondrial inner membrane
• mitochondrion
• oxidoreductase complex

Protein domainsUniProt · Pfam · InterPro

• Lactate/malate dehydrogenase, N-terminal
• L-lactate/malate dehydrogenase
• L-lactate dehydrogenase
• Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal
• L-lactate dehydrogenase, active site
• Lactate/malate dehydrogenase, C-terminal
• NAD(P)-binding domain superfamily
• lactate/malate dehydrogenase, NAD binding domain
• lactate/malate dehydrogenase, alpha/beta C-terminal domain

KeywordsUniProt

• Acetylation
• Cytoplasm
• Membrane
• Mitochondrion
• Mitochondrion inner membrane
• NAD
• Oxidoreductase
• Phosphoprotein

InteractionsUniProt · HPA

Protein binding partners of LDHB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MLDHR
• CTSD
• LDHA
• LDHC