LDHA

Protein identityUniProt · HPA

UniProt accession

P00338

Gene

LDHA

Ensembl

ENSG00000134333

Chromosome

11

Canonical length

332 aa

Protein class

Cancer-related genes, Disease related genes, Enzymes, FDA approved drug targets, Human disease related genes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Cytosol

Quaternary structure

Homotetramer

OverviewNCBI Gene

This gene encodes the A subunit of lactate dehydrogenase enzyme which catalyzes the reversible conversion of pyruvate to lactate with the concomitant oxidation of NADH to NAD in anaerobic glycolysis. The protein is found predominantly in skeletal muscle and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2023]

Canonical amino-acid sequenceUniProt

332 residues, UniProt reviewed canonical sequence.

>P00338|LDHA
     1  MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG
    61  EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI
   121  IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV
   181  HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE
   241  VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI
   301  SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against LDHA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

846 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about LDHA.

Disease | AllUniProt

Conditions LDHA is implicated in, by any mechanism.

• Glycogen storage disease 11 (GSD11) MIM:612933

Disease | GeneticClinVar

13 pathogenic / likely-pathogenic of 259 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Glycogen storage disease due to lactate dehydrogenase M-subunit deficiency

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.63

gnomAD pLI

0.07

gnomAD missense Z

1.58

DepMap mean gene effect

-0.4

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• glucose catabolic process to lactate via pyruvate
• glycolytic process
• lactate metabolic process
• pyruvate catabolic process
• substantia nigra development

Molecular functions

• cadherin binding
• identical protein binding
• L-lactate dehydrogenase (NAD+) activity

Cellular components

• cytosol
• extracellular exosome
• membrane
• mitochondrion
• nucleus
• oxidoreductase complex
• sperm fibrous sheath

Protein domainsUniProt · Pfam · InterPro

• Lactate/malate dehydrogenase, N-terminal
• L-lactate/malate dehydrogenase
• L-lactate dehydrogenase
• Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal
• L-lactate dehydrogenase, active site
• Lactate/malate dehydrogenase, C-terminal
• NAD(P)-binding domain superfamily
• lactate/malate dehydrogenase, NAD binding domain
• lactate/malate dehydrogenase, alpha/beta C-terminal domain

KeywordsUniProt

• Acetylation
• Cytoplasm
• Glycogen storage disease
• Isopeptide bond
• NAD
• Oxidoreductase
• Phosphoprotein
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of LDHA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• LDHB
• LDHC