Seroatlas · Human Serome Atlas

KLHL35

Kelch-like protein 35

Also known as: FLJ33790, KLH35_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q6PF15
Gene
KLHL35
Ensembl
ENSG00000149243
Chromosome
11
Canonical length
583 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nucleoli fibrillar center,Centrosome

OverviewNCBI Gene

Predicted to enable ubiquitin-like ligase-substrate adaptor activity. Predicted to be involved in proteasome-mediated ubiquitin-dependent protein catabolic process. Predicted to be part of Cul3-RING ubiquitin ligase complex. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

583 residues, UniProt reviewed canonical sequence.

>Q6PF15|KLHL35
     1  MRQGHAPEES EPGCEAPCAG PCHAQRVLQA LNAYRRSGTL TDVVLRAGGR DFPCHRAALS
    61  AGSAYFRSLF AAGRPERGPA VVPVVPVAPE APGTSPAGAA AALAVVLDYV YGAGVRLRAE
   121  DEAAAVLALA ERLGVAGLRE ACVRFLEGRL RAANSLALRR VAAAFSLAPL AERCGRVLRQ
   181  AFAEVARHAD FLELAPDEVV ALLADPALGV AREEAVFEAA MRWVRHDAPA RRGQLRRLLE
   241  HVRLPLLAPA YFLEKVEADE LLQACGECRP LLLEARACFI LGREAGALRT RPRRFMDLAE
   301  VIVVIGGCDR KGLLKLPFAD AYHPESQRWT PLPSLPGYTR SEFAACALRN DVYVSGGHIN
   361  SHDVWMFSSH LHTWIKVASL HKGRWRHKMA VVQGQLFAVG GFDGLRRLHS VERYDPFSNT
   421  WAAAAPLPEA VSSAAVASCA GKLFVIGGAR QGGVNTDKVQ CFDPKEDRWS LRSPAPFSQR
   481  CLEAVSLEDT IYVMGGLMSK IFTYDPGTDV WGEAAVLPSP VESCGVTVCD GKVHILGGRD
   541  DRGESTDKVF TFDPSSGQVE VQPSLQRCTS SHGCVTIIQS LGR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against KLHL35 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.27
Highest tissue expression
10 nTPM

Expression across tissuesHPA

Tissue

  • basal ganglia: 10 nTPM
  • testis: 9 nTPM
  • hypothalamus: 6 nTPM
  • cerebral cortex: 4.9 nTPM
  • spinal cord: 4.8 nTPM
  • hippocampal formation: 3.8 nTPM

Single-cell type

  • undifferentiated spermatogonia: 74 nCPM
  • oocytes: 60 nCPM
  • differentiating spermatogonia: 47 nCPM
  • late spermatids: 39 nCPM
  • platelets: 27 nCPM
  • retinal bipolar cells: 26 nCPM

Immune cell

  • basophil: 2.6 nTPM
  • gdT-cell: 0.8 nTPM
  • memory CD4 T-cell: 0.4 nTPM
  • total PBMC: 0.4 nTPM
  • myeloid DC: 0.3 nTPM
  • MAIT T-cell: 0.2 nTPM

Brain region

  • pons: 9.2 nTPM
  • hypothalamus: 8.7 nTPM
  • basal ganglia: 8.5 nTPM
  • white matter: 6.5 nTPM
  • cerebral cortex: 6.4 nTPM
  • midbrain: 5.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.9
gnomAD pLI
0
gnomAD missense Z
-0.15
DepMap mean gene effect
0.06
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads KLHL35 as an antibody target. Whether an autoantibody or antibody against KLHL35 could matter depends on whether native KLHL35 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

KLHL35 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label KLHL35 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/KLHL35. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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