Seroatlas · Human Serome Atlas

KCMF1

E3 ubiquitin-protein ligase KCMF1

Also known as: DEBT91, DKFZP434L1021, KCMF1_HUMAN, PCMF, ZZZ1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9P0J7
Gene: KCMF1
Ensembl: ENSG00000176407
Chromosome: 2
Canonical length: 381 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

Enables ubiquitin protein ligase activity. Involved in several processes, including negative regulation of HRI-mediated signaling; proteasome-mediated ubiquitin-dependent protein catabolic process; and protein polyubiquitination. Located in cytosol. Is active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

381 residues, UniProt reviewed canonical sequence.

>Q9P0J7|KCMF1
     1  MSRHEGVSCD ACLKGNFRGR RYKCLICYDY DLCASCYESG ATTTRHTTDH PMQCILTRVD
    61  FDLYYGGEAF SVEQPQSFTC PYCGKMGYTE TSLQEHVTSE HAETSTEVIC PICAALPGGD
   121  PNHVTDDFAA HLTLEHRAPR DLDESSGVRH VRRMFHPGRG LGGPRARRSN MHFTSSSTGG
   181  LSSSQSSYSP SNREAMDPIA ELLSQLSGVR RSAGGQLNSS GPSASQLQQL QMQLQLERQH
   241  AQAARQQLET ARNATRRTNT SSVTTTITQS TATTNIANTE SSQQTLQNSQ FLLTRLNDPK
   301  MSETERQSME SERADRSLFV QELLLSTLVR EESSSSDEDD RGEMADFGAM GCVDIMPLDV
   361  ALENLNLKES NKGNEPPPPP L

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against KCMF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.53
Highest tissue expression: 130 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 130 nTPM
pancreas: 75 nTPM
tongue: 67 nTPM
testis: 64 nTPM
bone marrow: 57 nTPM
esophagus: 55 nTPM

Single-cell type

neutrophils: 426 nCPM
esophageal apical cells: 391 nCPM
endometrial glandular cells: 347 nCPM
ocular epithelial cells: 300 nCPM
endometrial luminal cells: 267 nCPM
urothelial cells: 247 nCPM

Immune cell

T-reg: 6 nTPM
eosinophil: 5.4 nTPM
neutrophil: 4.5 nTPM
basophil: 3.1 nTPM
naive B-cell: 3.1 nTPM
gdT-cell: 2.7 nTPM

Brain region

cerebral cortex: 72 nTPM
thalamus: 69 nTPM
midbrain: 67 nTPM
medulla oblongata: 63 nTPM
hippocampal formation: 63 nTPM
pons: 63 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.3
gnomAD pLI: 0.98
gnomAD missense Z: 2.68
DepMap mean gene effect: -0.97
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of KCMF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads KCMF1 as an antibody target. Whether an autoantibody or antibody against KCMF1 could matter depends on whether native KCMF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

KCMF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label KCMF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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