Seroatlas · Human Serome Atlas

ITK

Tyrosine-protein kinase ITK/TSK

Also known as: EMT, ITK_HUMAN, LYK, PSCTK2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q08881
Gene: ITK
Ensembl: ENSG00000113263
Chromosome: 5
Canonical length: 620 aa
Protein class: Cancer-related genes, Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins
Subcellular location: Cytosol
Quaternary structure: Homooligomer

OverviewNCBI Gene

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

620 residues, UniProt reviewed canonical sequence.

>Q08881|ITK
     1  MNNFILLEEQ LIKKSQQKRR TSPSNFKVRF FVLTKASLAY FEDRHGKKRT LKGSIELSRI
    61  KCVEIVKSDI SIPCHYKYPF QVVHDNYLLY VFAPDRESRQ RWVLALKEET RNNNSLVPKY
   121  HPNFWMDGKW RCCSQLEKLA TGCAQYDPTK NASKKPLPPT PEDNRRPLWE PEETVVIALY
   181  DYQTNDPQEL ALRRNEEYCL LDSSEIHWWR VQDRNGHEGY VPSSYLVEKS PNNLETYEWY
   241  NKSISRDKAE KLLLDTGKEG AFMVRDSRTA GTYTVSVFTK AVVSENNPCI KHYHIKETND
   301  NPKRYYVAEK YVFDSIPLLI NYHQHNGGGL VTRLRYPVCF GRQKAPVTAG LRYGKWVIDP
   361  SELTFVQEIG SGQFGLVHLG YWLNKDKVAI KTIREGAMSE EDFIEEAEVM MKLSHPKLVQ
   421  LYGVCLEQAP ICLVFEFMEH GCLSDYLRTQ RGLFAAETLL GMCLDVCEGM AYLEEACVIH
   481  RDLAARNCLV GENQVIKVSD FGMTRFVLDD QYTSSTGTKF PVKWASPEVF SFSRYSSKSD
   541  VWSFGVLMWE VFSEGKIPYE NRSNSEVVED ISTGFRLYKP RLASTHVYQI MNHCWKERPE
   601  DRPAFSRLLR QLAEIAESGL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ITK can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.28
Highest tissue expression: 54 nTPM

Expression across tissuesHPA

Tissue

thymus: 54 nTPM
lymph node: 27 nTPM
tonsil: 23 nTPM
appendix: 15 nTPM
spleen: 9.8 nTPM
gallbladder: 4 nTPM

Single-cell type

t-cells: 564 nCPM
nk-cells: 295 nCPM
innate lymphoid cells: 136 nCPM
mast cells: 125 nCPM
early spermatids: 74 nCPM
thymocytes: 23 nCPM

Immune cell

naive CD4 T-cell: 78 nTPM
T-reg: 70 nTPM
naive CD8 T-cell: 64 nTPM
memory CD4 T-cell: 56 nTPM
memory CD8 T-cell: 54 nTPM
total PBMC: 44 nTPM

Brain region

thalamus: 0.1 nTPM
white matter: 0.1 nTPM
amygdala: 0 nTPM
basal ganglia: 0 nTPM
cerebellum: 0 nTPM
cerebral cortex: 0 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ITK.

Disease | AllUniProt

Conditions ITK is implicated in, by any mechanism.

Lymphoproliferative syndrome 1 (LPFS1) MIM:613011

Disease | GeneticClinVar

22 pathogenic / likely-pathogenic of 621 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Lymphoproliferative syndrome 1
Inborn genetic diseases

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.71
gnomAD pLI: 0
gnomAD missense Z: 1.53
DepMap mean gene effect: 0.02
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Protein kinase domain
SH2 domain
Serine-threonine/tyrosine-protein kinase, catalytic domain
SH3 domain
Zinc finger, Btk motif
Pleckstrin homology domain
Tyrosine-protein kinase, active site
Protein kinase-like domain superfamily
PH-like domain superfamily
Protein kinase, ATP binding site
Tyrosine-protein kinase, catalytic domain
SH3-like domain superfamily
SH2 domain superfamily
Non-receptor tyrosine kinases involved in cell signaling
SH2 domain
SH3 domain
PH domain
BTK motif
Protein tyrosine and serine/threonine kinase
Tyrosine-protein kinase ITK, SH3 domain
Tyrosine-protein kinase ITK/TSK, catalytic domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ITK in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ITK as an antibody target. Whether an autoantibody or antibody against ITK could matter depends on whether native ITK is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ITK is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ITK as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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