Seroatlas · Human Serome Atlas

THEMIS

Protein THEMIS

Also known as: bA325O24.3, bA325O24.4, C6orf190, C6orf207, FLJ40584, THMS1_HUMAN, TSEPA

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N1K5
Gene: THEMIS
Ensembl: ENSG00000172673
Chromosome: 6
Canonical length: 641 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Canonical amino-acid sequenceUniProt

641 residues, UniProt reviewed canonical sequence.

>Q8N1K5|THEMIS
     1  MALSLEEFVH SLDLRTLPRV LEIQAGIYLE GSIYEMFGNE CCFSTGEVIK ITGLKVKKII
    61  AEICEQIEGC ESLQPFELPM NFPGLFKIVA DKTPYLTMEE ITRTIHIGPS RLGHPCFYHQ
   121  KDIKLENLII KQGEQIMLNS VEEIDGEIMV SCAVARNHQT HSFNLPLSQE GEFYECEDER
   181  IYTLKEIVEW KIPKNRTRTV NLTDFSNKWD STNPFPKDFY GTLILKPVYE IQGVMKFRKD
   241  IIRILPSLDV EVKDITDSYD ANWFLQLLST EDLFEMTSKE FPIVTEVIEA PEGNHLPQSI
   301  LQPGKTIVIH KKYQASRILA SEIRSNFPKR HFLIPTSYKG KFKRRPREFP TAYDLEIAKS
   361  EKEPLHVVAT KAFHSPHDKL SSVSVGDQFL VHQSETTEVL CEGIKKVVNV LACEKILKKS
   421  YEAALLPLYM EGGFVEVIHD KKQYPISELC KQFRLPFNVK VSVRDLSIEE DVLAATPGLQ
   481  LEEDITDSYL LISDFANPTE CWEIPVGRLN MTVQLVSNFS RDAEPFLVRT LVEEITEEQY
   541  YMMRRYESSA SHPPPRPPKH PSVEETKLTL LTLAEERTVD LPKSPKRHHV DITKKLHPNQ
   601  AGLDSKVLIG SQNDLVDEEK ERSNRGATAI AETFKNEKHQ K

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against THEMIS can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.35
Highest tissue expression: 29 nTPM

Expression across tissuesHPA

Tissue

thymus: 29 nTPM
lymph node: 7.9 nTPM
tonsil: 4.2 nTPM
appendix: 2.8 nTPM
small intestine: 2.7 nTPM
cerebral cortex: 2.6 nTPM

Single-cell type

t-cells: 130 nCPM
oligodendrocytes: 15 nCPM
brain excitatory neurons: 12 nCPM
b-cells: 5.3 nCPM
nk-cells: 5.2 nCPM
proximal tubule cells: 2.2 nCPM

Immune cell

naive CD8 T-cell: 57 nTPM
memory CD8 T-cell: 49 nTPM
memory CD4 T-cell: 33 nTPM
MAIT T-cell: 32 nTPM
naive CD4 T-cell: 31 nTPM
gdT-cell: 27 nTPM

Brain region

cerebral cortex: 7.2 nTPM
white matter: 4.3 nTPM
medulla oblongata: 1.8 nTPM
basal ganglia: 1.7 nTPM
pons: 1.3 nTPM
midbrain: 1.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.87
gnomAD pLI: 0
gnomAD missense Z: -0.58
DepMap mean gene effect: 0.07
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of THEMIS in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads THEMIS as an antibody target. Whether an autoantibody or antibody against THEMIS could matter depends on whether native THEMIS is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

THEMIS is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label THEMIS as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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